香蕉NPR1基因家族的鉴定及在枯萎病菌胁迫下表达分析  

Genome-Wide Survey of the NPR1 Gene Family in Banana(Musa spp.) and Expression Pattern Analysis Upon Infection with Foc TR4

作　　者：任陪娣 谢碧玉 陈翠 贾彩红[1] 王静毅[1] 张建斌[1] 徐碧玉[1] 王卓[1] 金志强[1] REN Pei-di;XIE Bi-yu;CHEN Cui;JIA Cai-hong;WANG Jing-yi;ZHANG Jian-bin;XU Bi-yu;WANG Zhuo;JIN Zhi-qiang(Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Tropical Crop Biotechnology of the Ministry of Agriculture and Rural Affairs,Haikou 571101;College of Horticulture of Nanjing Agricultural University,Nanjing 210095)

机构地区：[1]中国热带农业科学院热带生物技术研究所/农业部热带生物技术重点开放实验室,海口571101 [2]南京农业大学园艺学院,南京210095

出　　处：《植物遗传资源学报》2019年第6期1621-1629,共9页Journal of Plant Genetic Resources

基　　金：国家自然科学基金(31501624);海南省自然科学基金资助项目(318MS090);现代农业产业技术体系(CARS-32)~~

摘　　要：以拟南芥NPR1基因家族成员蛋白序列为查询序列,在香蕉基因组数据库中鉴定香蕉NPR1基因家族成员,并对其进行生物信息学分析及在枯萎病菌侵染下的表达分析。共鉴定得到15个成员,将其命名为MaNPR1~MaNPR15。理化性质、保守功能结构域、重要的氨基酸残基及motif分析结果与其他物种所报道的有较高的一致度。香蕉NPR1基因家族成员种内进化树、基因结构及结构域的分类情况呈现出高度一致,表明了香蕉NPR1基因家族成员间有着明确的分工。种间进化树显示香蕉的NPR1基因分为3个分组,每个分组都含有拟南芥NPR1成员。主栽品种巴西蕉(Musa acuminata Colla.AAA group'Brazilian')易感香蕉枯萎病,从巴西蕉CK及巴西蕉受枯萎病侵染2 d的根系转录组数据中综合表达量及表达趋势选定8个成员,并在巴西蕉与抗(耐)Foc TR4品种GCTCV-119中验证其表达模式。基因MaNPR4及MaNPR11在抗感品种中表现出明显的差异表达,在抗病品种GCTCV-119中随着Foc TR4侵染时间延长表达量不断增加,而在感病品种巴西蕉中表达量呈下降趋势。表明MaNPR4及MaNPR11参与香蕉抗枯萎病过程。本研究结果为进一步利用香蕉NPR1基因进行香蕉抗枯萎病遗传改良奠定基础。Using the NPR1 gene family member's protein sequences from Arabidopsis Heynh.as query,MaNPR1 gene family members were identified in the banana A genome database,and these features using bioinformatics methods and the expression upon infection with Foc TR4 were further investigated.In total,15 family members were identified,designated MaNPR1-MaNPR15.Physicochemical properties,conserved functional domain,essential amino acid residues and motif analysis results of MaNPR1 gene family are highly consistent with those of other species.The classification of phylogenetic tree,gene structure and functional domain suggested a functional specificity among members of the MaNPR1 gene family.The interspecific phylogenetic tree showed that the MaNPR1 gene family were divided into three subgroups,co-residing with respective Arabidopsis Heynh.NPR1 members in each group.The predominant-cultivating banana variety'Cavendish'(Musa acuminata Colla.AAA group)was highly susceptible to fusarium wilt caused by Fusarium oxysporum f.sp.Cubense Tropical Race 4(Foc TR4).By analyzing the transcriptome dataset from root transcriptome data of infected and un-infected Brazilian samples,eight differentially expressed MaNPR1 genes were identified,which were further tested by RT-qPCR in'Cavendish'and the resistant variety'GCTCV-119'.Two members MaNPR4 and MaNPR11 showed an increasing transcriptional levels at time-points in'GCTCV-119',in relative to the constant reduction in'Cavendish'.That indicated an involvement of MaNPR4 and MaNPR11 in responses to fusarium wilt.Taken together,this study raised a potential in breeding for fusarium wilt resistance banana by use of NPR1 genes.

关 键 词：香蕉 NPR1 生物信息学分析 香蕉枯萎病 表达分析 

分 类 号：S43[农业科学—农业昆虫与害虫防治]