试验相关因素对SD大鼠临床生化学指标的影响  

Effects of related factors on clinical biochemical parameters in SD rats

作　　者：靳苏香 程洁[2] 钱雯 严婷 秦珩[1,2] 章婉 董昊[1] 张璐璐 王玉邦 环飞 JIN Su-xiang;CHENG Jie;QIAN Wen;YAN Ting;QIN Heng;ZHANG Wan;DONG Hao;ZHANG Lu-lu;WANG Yu-bang;HUAN Fei(The Safety Assessment and Research Center for Drugs of Jiangsu Province,Nanjing Jiangsu 211166,China;School of Public Health Nanjing Medical University,Nanjing Jiangsu 211166,China)

机构地区：[1]江苏省医药农药兽药安全性评价与研究中心,江苏南京211166 [2]南京医科大学公共卫生学院,江苏南京211166

出　　处：《毒理学杂志》2019年第5期366-369,380,共5页Journal of Toxicology

基　　金：江苏高校优势学科建设工程资助项目(PAPD)

摘　　要：目的研究试验相关因素对SD大鼠临床生化学指标的影响。方法选择SPF级SD大鼠为实验动物,按性别(A)、停食(B)、麻醉剂(C)和动静脉采血(D) 4个因素,根据不同因素交互影响分为24组,采集各组SD大鼠血液标本,测定血清丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)、总蛋白(TP)、白蛋白(ALB)、总胆红素(TBIL)、碱性磷酸酶(ALP)、血糖(GLU)、尿素氮(BUN)、肌酐(CREA)、总胆固醇(CHOL)、甘油三酯(TG)和胆碱酯酶(CHE)等指标。结果性别(A)对TP、ALB和BUN无影响,而对其余9项生化指标有明显影响,差异有统计学意义(P<0. 05);停食(B)对ALT、AST、TP、ALB、CREA和CHE无影响,而对TBIL、ALP、GLU、BUN、CHOL和TG有明显影响(P<0. 05);麻醉剂(C)仅对TBIL无影响,对其他11项生化指标均有影响(P<0. 05);动静脉采血(D)对ALP、TP和ALB有明显影响(P<0. 05),而其余9项生化指标均无影响。结论 SD大鼠大部分临床生化学指标存在性别、停食、麻醉剂以及动静脉采血差异。Objective To study the effects of related factors on blood biochemical parameters in SD rats. Methods According to gender(A),fasting time(B),anesthesia(C) and blood from arteries and veins(D),SPF-grade SD rats were divided into 24 groups.Blood samples were collected from each group. Then ALT,AST,TP,ALB,TBIL,ALP,GLU,BUN,CREA,CHOL,TG and CHE were measured. Result There was significant effect of gender(A) on nine biochemical parameters except for TP,ALB and BUN(P<0. 05).There was sigmificant effect of fasting(B) on TBIL,ALP,GLU,BUN,CHOL and TG except for ALT,AST,TP,ALB,CREA and CHE(P < 0. 05). There was significant effect of anesthesia on all biochemical parameters except for TBIL(P<0. 05). There was significant effect of blood collection mode(D) on ALP,TP and ALB except for the other nine biochemical parameters(P < 0. 05).Conclusion Most of the clinical biochemical parometers of SD rats showed differences in gender,fasting time,anesthesia and blood from arteries and veins.

关 键 词：SD大鼠 临床生化学指标 麻醉剂 

分 类 号：Q95-33.285636[生物学—动物学] R114[医药卫生—卫生毒理学]