露兜树感染黄瓜花叶病毒的鉴定  

Identification of Cucumber mosaic virus Infecting Pandanus tectorius

作　　者：王凯利 张洁[2] 吴阔[2] 杨学宝 魏建丽 王昌命 董家红[2,4] WANG Kaili;ZHANG Jie;WU Kuo;YANG Xuebao;WEI Jianli;WANG Changming;DONG Jiahong(Southwest Forestry University,College of Life Sciences,Kunming 650224,China;Biotechnology and Germplasm Resources Institute,Yunnan Academy of Agricultural Sciences,Kunming 650223,China;Yuanjiang County Agricultural Technology Extension Station,Yuxi 653100,China;College of Traditional Chinese Pharmacy,Yunnan University of Chinese Medicine,Kunming 650500,China)

机构地区：[1]西南林业大学生命科学学院,昆明650224 [2]云南省农业科学院生物技术与种质资源研究所,昆明650223 [3]元江县农业技术推广站,云南玉溪653100 [4]云南中医药大学中药学院,昆明650500

出　　处：《园艺学报》2019年第10期2055-2060,共6页Acta Horticulturae Sinica

基　　金：云南省方荣祥院士工作站;云南省应用基础研究计划重点项目(2017FA019)

摘　　要：为了确定引起云南元江露兜树(Pandanus tectorius)叶片黄化、花叶斑点的病毒病原。取症状明显的叶片进行负染,经电镜观察到病毒粒子形态为球形,直径约为30 nm。使用黄瓜花叶病毒外壳蛋白基因序列特异性引物,对露兜树上的病毒分离物进行病毒基因RT-PCR扩增分析,克隆、测序目的片段,发现其与印度、中国四川、泰国的黄瓜花叶病毒分离物的外壳蛋白基因序列相似性为98%,属于CMV亚组Ⅰ。这是露兜树被病毒感染的首次报道。In order to confirm the viral pathogen causing yellowing and mosaic spots symptoms in the leaves of Pandanus tectorius in Yuanjiang County,Yunnan Province.Electron microscopy revealed that the virus in the dips of the diseased leaves has icosahedral particles ca.30 nm in diameter.With the specific primer pairs for coat protein(CP)gene sequence of Cucumber mosaic virus(CMV),RT-PCR was used to detect the viral pathogen infection to P.tectorius and the target fragment was cloned and sequenced.The sequence analysis showed that it had the highest identity of 98% with CP gene sequence of CMV isolates from Indian,Sichuan and Thailand.This isolate belongs to CMV subgroup I.It is the first report of infection of P.tectorius by a viral pathogen.

关 键 词：露兜树 黄瓜花叶病毒 病毒粒子 外壳蛋白基因 侵染 

分 类 号：S68[农业科学—观赏园艺]