类器官和立体细胞模型在中药心脏毒性评价中的应用前景  

Application prospect of organoids and 3D-cell models in evaluation of cardiotoxicity of traditional Chinese medicine

作　　者：刘昕彦 邵瑞[1,2] 贺爽[1,2] 朱彦[1,2] LIU Xin-yan;SHAO Rui;HE Shuang;ZHU Yan(Tianjin Modern Chinese Medicine Key Laboratory,Tianjin University of Traditional Chinese Medicine,Tianjin 300193,China;Tianjin International Biomedical Research Institute,Chinese Medicine New Drug Research and Development Center,Tianjin 300457,China)

机构地区：[1]天津中医药大学,天津市现代中药重点实验室,天津300193 [2]天津国际生物医药联合研究院中药新药研发中心,天津300457

出　　处：《药学学报》2019年第11期1888-1894,共7页Acta Pharmaceutica Sinica

基　　金：国家重点研发计划项目(2018YFC1704500);国家科技重大专项(2018ZX01031301);国家自然科学基金资助项目(81873037)

摘　　要：心脏毒性是导致药物研发失败和上市后撤市的原因之一。然而,目前的药物心脏毒性评价方法存在临床相关性低、重现性低、处理量小等缺点。建立准确可靠的方法评价药物的心脏毒性是当前药物安全性评价及毒理学研究亟需解决的问题。心脏类器官作为新一代的药物心脏毒性评价模型,极大程度保留了心脏细胞在体内的生物特性和功能,更加真实、准确地反映药物对心脏的影响。本文就心脏立体细胞模型和类器官体外培养技术的进展作一综述,并重点讨论心脏类器官在中药心脏毒性评价中的应用及其发展潜力。文章还讨论了细胞和类器官模型在面对中药心脏毒性评价独特挑战方面的优势和前景。Cardiotoxicity is one of the main causes of failure in new drug development or drug withdrawal from the market.However,current methods for evaluation of drug cardiotoxicity suffer the shortcomings such as low clinical relevance,low reproducibility and lack of high throughput screening capacity.Therefore,there is an urgent need for establishing more accurate and reliable methods for cardiotoxicity evaluation of drugs.As a new generation of drug cardiotoxicity evaluation,cardiac organs in culture retain the biological characteristics and functions of heart cells in the body,and can realistically and accurately respond to the effects of drugs.This article reviews recent progress of in vitro culture of cardiac organs and 3 D-cell models,with focuses on application and development potential of cardiac organs for evaluation of cardiotoxicity of traditional Chinese medicine.The advantage and future prospective of such cell-and organ-based models for unique challenges in evaluation of cardiotoxicity of traditional Chinese medicine have been discussed.

关 键 词：中药 心脏毒性 二维细胞 三维细胞 类器官 毒性评价技术 

分 类 号：R285[医药卫生—中药学]