红树林来源耐酸真菌Aspergillus fumigatus OUCMDZ-5210次生代谢产物的研究  

Studies on secondary metabolite of aciduric fungus Aspergillus fumigatus OUCMDZ-5210 derived from Thailand mangrove

作　　者：王亚楠 高海 丁健 王乂[1,2] 付鹏 朱伟明 WANG Ya-nan;GAO Hai;DING Jian;WANG Yi;FU Peng;ZHU Wei-ming(School of Medicine and Pharmacy,Ocean University of China,Qingdao 266003,China;Laboratory for Marine Drugs and Bioproducts of Qingdao Pilot National Laboratory for Marine Science and Technology,Qingdao 266237,China)

机构地区：[1]中国海洋大学医药学院,山东青岛266003 [2]青岛海洋科学与技术试点国家实验室海洋药物与生物制品功能实验室,山东青岛266237

出　　处：《中国海洋药物》2019年第5期47-53,共7页Chinese Journal of Marine Drugs

基　　金：国家自然科学基金项目（81973198,U1501221,81561148012,U1606403）;山东省重点研发计划项目（2018GSF118070）资助

摘　　要：目的对源自泰国红树林底泥中的耐酸真菌Aspergillus fumigatus OUCMDZ-5210的次生代谢产物进行化学成分和生物活性的研究。方法综合利用硅胶柱色谱、凝胶柱色谱和半制备高效液相色谱(HPLC)等多种方法对耐酸真菌Aspergillus fumigatus OUCMDZ-5210的次生代谢产物进行分离;采用核磁共振谱(NMR)、质谱(MS)、紫外光谱(UV)等现代波谱学技术对分离得到的化合物进行结构鉴定;以人慢性髓性白血病细胞K562及人结肠癌细胞HCT116两株肿瘤细胞为研究模型,采用MTT法和CCK-8法对所分离的化合物进行细胞毒活性评价。结果从耐酸真菌Aspergillus fumigatus OUCMDZ-5210的次级代谢产物中分离得到6个吲哚二酮哌嗪类生物碱:tryprostatins B (1),fumitremorgin C (2),spirotryprostatin A (3),spirotryprostatin.B(4),6-methoxyspirotryprostatin B(5),8,9-dihydroxyspirotrypr-ostatin A (6)以及1个酰胺类化合物cephalimysin B (7)。结论生物活性初步评价结果显示,化合物3和6对人结肠癌细胞HCT116以及人慢性髓性白血病细胞K562具有不同程度的抑制活性。Objective To find secondary metabolites with structural specificity and significant cytotoxic activity,the secondary metabolites of the aciduric fungus Aspergillus fumigatus OUCMDZ-5210,an aciduric fungus derived from Thai mangrove sediment and their bioactivities were investigated.Methods Using Silica gel column chromatography,Sephadex LH-20 and HPLC,the secondary metabolites of Aspergillus fumigatus OUCMDZ-5210 were isolated.The structures of those compounds were identified by several modern spectroscopy techniques including NMR,MS,UV.The cytotoxicities of those compounds against two cancer cell lines K562 and HCT-116 were evaluated by MTT and CCK-8 methods.Results Seven compounds were isolated from the secondary metabolites of Aspergillus fumigatus OUCMDZ-5210:tryprostatinsB(1),fumitremorginC(2),spirotryprostatinA(3),spirotryprostatinB(4),6-methoxyspirotryprostatinB(5),8,9-dihydroxyspirotryprostatinA(6),cep-halimysin B(7).Conclusion Preliminary evaluation of biological activity showed that compounds 3 and 6 had different degrees of inhibitory activities against human colon cancer cell HCT116 and human chronic myelogenous leukemia cell K562.

关 键 词：红树林真菌 次生代谢产物 细胞毒活性 

分 类 号：R915[医药卫生—微生物与生化药学]