妇科止带片中金胺O的检测方法研究  

Detection methods for auramine O in Fuke Zhidai tablets

作　　者：黄晓燕 孙辉 丁野 李文莉 李瑞莲 HUANG Xiao-yan;SUN Hui;DING Ye;LI Wen-li;LI Rui-lian(Hunan Institute for Drug Control,Changsha 410001;Hunan Engineering&Technology Research Center for Pharmaceutical Quality Evaluation,Changsha 410001)

机构地区：[1]湖南省药品检验研究院,长沙410001 [2]湖南省药品质量评价工程技术研究中心,长沙410001

出　　处：《中南药学》2019年第11期1906-1908,共3页Central South Pharmacy

基　　金：国家药典委员会国家药品标准提高行动计划项目(编号：36);湖南省食品药品监督管理局2018年食品药品安全科技项目(编号：湘食药科R201801)

摘　　要：目的建立高效液相色谱(HPLC-PDA)法和液相色谱-质谱联用(LC-MS/MS)法对妇科止带片中可能掺入的化工染料金胺O进行测定。方法采用HPLC-PDA法对妇科止带片样品进行筛查,采用LC-MS/MS法对筛查结果进行验证。结果市售的25批样品中,有5批检出了金胺O。结论建立的检查方法准确、可靠、专属性强,可有效检测妇科止带片中金胺O的染色情况。Objective To determine the inspect the chemical dye auramine O possibly mixed in Fuke Zhidai tablets by HPLC-PDA and LC-MS/MS.Methods HPLC-PDA was used to screen auramine O.While LC-MS/MS was used to verify the results.Results Twenty-five batches of samples were measured and auramine O was detected in 5 batches.Conclusion The methods are highly accurate,specific and reliable,which can be applied to detect auramine O in Fuke Zhidai tablets.

关 键 词：妇科止带片 金胺O 高效液相色谱法 液相色谱-质谱联用法 

分 类 号：R286[医药卫生—中药学] R927[医药卫生—中医学]