细胞外基质对背根神经节细胞生长分化的影响  

Effect of Extracellular Matrix on Growth and Differentiation of Dorsal Root Ganglion Cells

作　　者：戴宛平 潘瑶 康倩若 任雪敏 阳小飞[1] 刘孟雪 DAI Wanping;PAN Yao;KANG Qianruo;REN Xuemin;YANG Xiaofei;LIU Mengxue(Key Laboratory of Brain Cognition,State Civil Affairs Commission,School of Biomedical Engineering,Central South University for Nationalities,Key Laboratory ofMedical Information Analysis and Oncology Diagnosis and Treatment,Hubei Province,Laboratory ofMembrane Ion Channel and Drug Research and Development,Wuhan,Hubei 430074)

机构地区：[1]中南民族大学生物医学工程学院脑认知国家民委重点实验室医学信息分析及肿瘤诊疗湖北省重点实验室膜离子通道与药物研发实验室

出　　处：《科教导刊》2019年第29期81-83,共3页The Guide Of Science & Education

基　　金：国家自然科学基金项目(31670850);中南民族大学科学基金引进人才科研启动基金自科项目(ZZ13002)资助

摘　　要：背根神经节细胞(DRG)是外周神经系统中感受、传导痛觉的重要细胞,多聚赖氨酸是神经细胞体外培养常用的细胞外基质(ECM),但不同类型、不同浓度的多聚赖氨酸对背根神经节细胞生长分化的具体影响目前仍未阐明。目的:本项目旨在探讨不同类型、浓度、分子量的多聚赖氨酸对大鼠背根神经节细胞生长分化的影响。方法:分别从时间梯度、浓度梯度以及不同分子量这三个方面研究D型及L型多聚赖氨酸对大鼠背根神经节细胞分化率及平均突起长度的影响。结果:实验结果表明,正常工作浓度下用L型多聚赖氨酸作为基底材料培养细胞48 h时对细胞突起长度有一定的促进作用;当D型、L型多聚赖氨酸的浓度分别降低至5 g/ml、20 g/ml时,细胞在L型多聚赖氨酸包被的玻片上分化更好;而普通分子量(3-7 W)或高分子量(>30 W)的D型多聚赖氨酸对背根神经节细胞的生长分化无显著影响。Dorsal root ganglion cells(DRG)are important cells in the peripheral nervous system to sense and transmit pain.Polylysine is a common extracellular matrix(ECM)for nerve cells cultured in vitro.However,the specific effects of different types and concentrations of polylysine on the growth and differentiation of dorsal root ganglion cells have not yet been clarified.Objective:To investigate the effects of polylysine of different types,concentration and molecular weight on the growth and differentiation of rat dorsal root ganglion cells.Methods:The effects of D-type and L-type polylysine on the differentiation rate and average process length of rat dorsal root ganglion cells were studied from three aspects:time gradient,concentration gradient and molecular weight.Results:The results showed that L-polylysine could promote the length of cell processes at 48 hours in normal working concentration.When the concentration of D-polylysine and L-polylysine decreased to 5 ug/ml and 20 ug/ml respectively,the cells differentiated better on L-polylysine-coated slide,while the normal molecular weight(3-7 W)or high molecular weight(>3 ug/ml)were better.0 W)D-polylysine had no significant effect on the growth and differentiation of dorsal root ganglion cells.

关 键 词：细胞分化 背根神经节细胞 底物成分 多聚赖氨酸 

分 类 号：R338.1[医药卫生—人体生理学]