RNA-Seq在AM真菌研究中的应用  

RNA-Seq application in study of arbuscular mycorrhizal fungi

作　　者：邓杰 李芳[1] 张伟珍 段廷玉[1] DENG Jie;LI Fang;ZHANG Wei-zhen;DUAN Ting-yu(State Key Laboratory of Grassland Agro-ecosystems,Key Laboratory of Grassland Livestock Industry Innovation,Ministry of Agriculture and Rural Affairs,Engineering Research Center of Grassland Industry,Ministry of Education,College of Pastoral Agriculture Science and Technology,Lanzhou University,Lanzhou 730020,China)

机构地区：[1]兰州大学草地农业生态系统国家重点实验室农业农村部草牧业创新重点实验室草地农业教育部工程研究中心兰州大学草地农业科技学院

出　　处：《生态学杂志》2019年第11期3542-3550,共9页Chinese Journal of Ecology

基　　金：国家绿肥产业技术体系项目(CARS-22)资助

摘　　要：丛枝菌根(arbuscular mycorrhizas,AM)真菌是农业生态系统中重要的微生物成员之一,可与约80%的维管束植物建立共生关系,这种共生关系一直伴随植物的进化过程,是影响植物生长及多样性的关键因素。转录组测序技术已广泛用于植物-AM真菌共生互作中信号传导、代谢、蛋白合成等生物过程的分子机理的研究。本文归纳了AM真菌与植物共生过程中参与植物胁迫防御、蛋白合成、蛋白折叠和降解、能量代谢、信号转导、转录等相关功能基因研究进展,如磷酸盐转运蛋白、凝集素前体、谷胱甘肽硫-转移酶、Mtha1质膜ATP酶、核糖体蛋白等相关基因,总结了相关研究中基因功能特征。为进一步深入研究AM真菌与植物共生机制提供理论依据。Arbuscular mycorrhizal(AM)fungi are important microbial components in agroecosystems.These fungi establish symbiotic associations with nearly 80%of vascular plants.The symbiotic relationship accompanied by plant evolution is a key factor affecting plant growth and diversity.In recent years,RNA-Seq has been widely used to study the molecular mechanism of stress defense,metabolism,protein synthesis and other biological processes in AM fungi and plant symbionts.We summarized the research progress of functional genes involved in plant stress defense,protein synthesis,protein folding/degradation,energy metabolism,signal transduction,and transcription during symbiosis,including those differentially expressed genes related to symbiosis such as phosphate transporter,lectin precursor,glutathione S-transferases,Mtha1 plasma membrane ATPase,and ribosomal protein,and reveals their functional characteristics.These findings provide theoretical basis for further research on the mechanism of AM fungi-plant symbiosis.

关 键 词：AM真菌 农业生态系统 植物 共生 功能基因 

分 类 号：Q94[生物学—植物学]