白介素6通过阻断L型钙离子通道活性抑制急性胰腺炎大鼠结肠纵行肌条收缩  

IL-6 inhibits colonic longitudinal muscle contraction by inactivating L-type calcium channel in rats with pancreatitis

作　　者：唐亚 梁世伟 全晓静[2] 罗和生[2] 刘颖 TANG Ya;LIANG Shi-Wei;QUAN Xiao-Jing;LUO He-Sheng;LIU Ying(Department of Gastroenterology,The Second Affiliated Hospital of Guilin Medical University,Guilin 541100,China;Department of Gastroenterology,Renmin Hospital of Wuhan University,Wuhan 430060,China)

机构地区：[1]桂林医学院第二附属医院消化科,桂林541100 [2]武汉大学人民医院消化科,武汉430060

出　　处：《生理学报》2019年第5期717-724,共8页Acta Physiologica Sinica

基　　金：supported by the National Natural Science Foundation of China(No.81660097,81460111)

摘　　要：本文旨在探讨白介素6 (interleukin 6, IL-6)对急性胰腺炎(acute pancreatitis, AP)大鼠结肠纵行肌条收缩的作用及其机制。用雨蛙肽和脂多糖联合诱导法制备AP大鼠模型,用生物机能实验系统观察IL-6对大鼠结肠纵行平滑肌条自发性收缩的影响,用ELISA检测血清IL-6的水平,用免疫组织化学染色法观察IL-6在结肠的表达分布,用全细胞膜片钳技术观察IL-6对结肠平滑肌细胞L型钙离子通道的影响。结果显示,与对照组相比,AP组大鼠结肠平滑肌条收缩幅度显著减小(P <0.05),收缩周期延长(P <0.05);IL-6可延长大鼠结肠平滑肌条收缩周期,但对肌条的自发性收缩幅度无影响。AP组大鼠血清IL-6浓度明显高于对照组,差异具有显著性(P <0.01);对照组大鼠结肠中IL-6表达较弥散,而在AP组结肠腺体、黏膜及黏膜下层中IL-6表达明显增强。IL-6可显著降低大鼠结肠平滑肌细胞L型钙离子通道的峰电流密度。以上结果提示,AP大鼠结肠运动减弱,其机制可能是表达上调的IL-6阻断结肠平滑肌细胞L型钙离子通道活性,进而抑制结肠纵行平滑肌收缩。The aim of this study was to investigate the effect of interleukin 6(IL-6) on the contraction of colon longitudinal muscle strips in rats with acute pancreatitis(AP) and its underlying mechanism. Rat AP model was established by combined injection(i. p.) of ceruletide and lipopolysaccharide. The effect of IL-6 on spontaneous contraction of longitudinal smooth muscle strips of rat colon was observed by biological function experiment system. The level of serum IL-6 was detected by ELISA, the expression and distribution of IL-6 in colon were observed by histochemical staining, and the effect of IL-6 on L-type calcium channel in colon smooth muscle cells was observed by whole cell patch clamp technique. The results showed that, compared with the control group, AP group exhibited reduced contractile amplitude and longer contraction cycle of colon smooth muscle strips. IL-6 prolonged the contraction cycle of colon smooth muscle strips, but did not affect their spontaneous contraction amplitude. Serum IL-6 concentration in AP group was significantly higher than that in control group(P < 0.05). IL-6 was diffusely distributed in the colon of the control group, but the expression of IL-6 was significantly up-regulated in the colon gland, mucosa and submucosa of the AP group. IL-6 significantly decreased the peak current density of L-type calcium channel in rat colon smooth muscle cells. These results suggest that the colon motility of AP rats is weakened, and the mechanism may be that up-regulated IL-6 inactivates L-type voltage-dependent calcium channels, and then inhibits the contraction of colon longitudinal smooth muscle.

关 键 词：急性胰腺炎 白介素6 平滑肌细胞 L型钙离子通道 膜片钳 

分 类 号：R657.5[医药卫生—外科学]