HPLC法同时测定伤痛克酊中3种姜黄素的含量  

Simultaneous Determination of Three Curcumins in Shangtongkeding by HPLC

作　　者：覃彪 邓明启[1] 黎敏 林锦良 唐承性 QIN Biao;DENG Ming-qi;LI Min;LIN Jin-liang;TANG Cheng-xing(College of Chemistry and Chemical Engineering,Zunyi Normal University,Zunyi 563006,China;Key Laboratory of Utilization Research on Characteristic Resources in Qianbei,Zunyi Normal University,Zunyi 563006,China)

机构地区：[1]遵义师范学院化学化工学院,贵州遵义563006 [2]遵义师范学院黔北特色资源应用研究实验室,贵州遵义563006

出　　处：《化学试剂》2019年第11期1124-1127,共4页Chemical Reagents

基　　金：遵义市红花岗区科学技术项目(遵红科合社字(2015)16号);遵义师范学院博士基金资助项目(遵师BS[2014]11号);遵义市科技局、遵义师范学院联合科技研发资金资助项目(遵市科合计[2018]05号)

摘　　要：建立HPLC法同时测定苗药伤痛克酊中姜黄素、去甲氧基姜黄素和双去甲氧基姜黄素的含量。以伤痛克酊为酊剂,分析采用Shimadzu Inert Sustain C18色谱柱(4. 6 mm×250 mm×5μm);以9. 6%冰醋酸水溶液-乙腈为流动相,梯度洗脱;检测波长为254 nm,流速为1. 0 m L/min,柱温为30℃。3种姜黄素分别在各自范围内线性关系良好(r≥0. 999 95),平均加样回收率分别为100. 26%、101. 27%、99. 07%,RSD分别为0. 13%、0. 11%、0. 32%。方法简便快捷,可用于伤痛克酊的质量控制。To establish the HPLC method for simultaneous determination of three curcumins in Shangtongkeding.Shangtongkeding is methanol tincture and the analysis of this drug was performed on the 30℃thermostatic Shimadzu InertSustain C18 column(4.6 mm×250 mm×5μm),with the mobile phase comprising of acetonitrile-water(containing 9.6%Glacial acetic acid)flowing at 1.0 mL/min in a gradient elution way,and the detection wavelength was set at 254 nm.Bisdethoxycurcumin,demethoxycurcumin and curcumin showed good linear relationships within the ranges,whose average recoveries were 99.07%,101.27%,100.26%with the RSDs of 0.32%,0.11%,0.13%,respectively.The method can be used for the quality control of Shangtongkeding.

关 键 词：HPLC法 伤痛克酊 姜黄素 去甲氧基姜黄素 双去甲氧基姜黄素 

分 类 号：O6[理学—化学]