不同激素对青钱柳外植体和愈伤组织褐化的影响  

The Effect of Different Hormones on Explant Browning and Callus Browning in Cyclocarya paliurus

作　　者：冯莹[1] 钱莲文[1] 林庆良[2] Ying Feng;Lianwen Qian;Qingliang Lin(School of Resource and Environmental Science,Quanzhou Normal University,Quanzhou 362000,China;Fujian Agriculture and Forestry University,Fuzhou 350002,China)

机构地区：[1]泉州师范学院资源与环境学院,泉州362000 [2]福建农林大学,福州350002

出　　处：《植物学报》2019年第5期634-641,共8页Chinese Bulletin of Botany

基　　金：福建省科技厅引导性项目(No.2017N0028);泉州市科技计划(No.2018N007、No.2016N002);泉港区科技计划(No.2016N06)

摘　　要：以青钱柳(Cyclocarya paliurus)叶片为研究材料,采用单因素完全随机试验法比较不同激素(6-BA、GA3和NAA)浓度组合对叶片外植体褐化的影响;在此基础上,分别采用二因素、单因素完全随机试验法比较6-BA+NAA不同浓度组合、不同基本培养基对愈伤组织褐化的影响。结果表明,改良MS+1.0 mg·L^–16-BA+1.0 mg·L^–1 GA3+0.3 mg·L^–1 NAA是抑制青钱柳叶片外植体褐化的最佳培养基,其褐化率为0,愈伤组织诱导率100%;改良MS+0.5 mg·L^–16-BA+0.2 mg·L^–1 NAA是抑制愈伤组织褐化的最佳培养基,其褐化率为0,愈伤组织增殖倍数达4.80倍,愈伤组织呈黄绿色或黄色,颗粒状,颗粒小而紧密、质硬且表面干燥。该方法有效解决了青钱柳叶片外植体和愈伤组织褐化问题,为青钱柳组织培养褐化控制提供了一条简单高效的离体培养途径,也为青钱柳叶片离体再生体系的建立奠定基础。To determine the effect of different hormones on explant browning and callus browning in Cyclocarya paliurus,leaf browning was controlled on medium with different kinds and content of hormones(6-BA,GA3,NAA)by a single-factor completely random test.Callus browning was controlled on the medium with different content of 6-BA+NAA and different basic medium by a double-and single-factor completely random test,respectively.The rate of leaf browning reached 0,and the rate of callus induction was up to 100%on the improved MS medium with 1.0 mg·L^–16-BA and 1.0 mg·L^–1 GA3 and 0.3 mg·L^–1 NAA.The rate of callus browning reached 0,and the callus propagation was up to 4.80 times on the improved MS medium with 0.5 mg·L^–16-BA and 0.2 mg·L^–1 NAA.The callus was yellow with green or yellow,granular,small and compact,hard and dry.This system effectively solved the problem of leaf browning and callus browning and provided a simple and effective way for tissue culture and a solid foundation for the regeneration system from leaf of C.paliurus.

关 键 词：褐化 青钱柳 愈伤组织 诱导 增殖 

分 类 号：S68[农业科学—观赏园艺]