一株广盐性蛭弧菌的生物学特性  

Characterization of a euryhaline Bdellovibrio-and-like organism

作　　者：陈丹 吴冰 韩红操[3] 蔡俊鹏 CHEN Dan;WU Bing;HAN Hong-Cao;CAI Jun-Peng(School of Food Science and Engineering,South China University of Technology,Guangzhou,Guangdong 510641,China;Analytical and Testing Center,South China University of Technology,Guangzhou,Guangdong 510640,China;School of Biology and Biological Engineering,South China University of Technology,Guangzhou,Guangdong 510006,China)

机构地区：[1]华南理工大学食品科学与工程学院,广东广州510641 [2]华南理工大学分析测试中心,广东广州510640 [3]华南理工大学生物科学与工程学院,广东广州510006

出　　处：《微生物学通报》2019年第11期3030-3039,共10页Microbiology China

基　　金：广东省科技计划公益项目(2016A020222002)~~

摘　　要：【背景】蛭弧菌有裂解水产养殖中常见致病菌的能力,具有重要的潜在应用价值,但在实际应用中,存在着菌株生长条件与应用环境不相符而导致效果差乃至无效果等问题。因此获得适应范围宽的蛭弧菌甚为关键。【目的】筛选出一株广盐性蛭弧菌以利推广应用;提升蛭弧菌蛭质体密度以利保存。【方法】以枯草芽孢杆菌为宿主,于浅滩水域分离纯化出一株广盐性蛭弧菌;对该广盐性蛭弧菌菌株进行分子生物学鉴定;之后探究其生物学特性及裂解性能,并研究谷氨酸钠、Ca2+和Mg2+、吲哚等影响蛭质体密度的因素。【结果】分离获得一株广盐性蛭弧菌BDN-1,其适宜温度、盐度和pH范围分别为20-30°C、0.5%-3.0%、6.0-8.5;BDN-1对30株受试菌的裂解率为86.7%,对其中16株受试弧菌裂解率为87.5%;谷氨酸钠、吲哚、Ca2+和Mg2+对BDN-1蛭质体密度有提升作用。【结论】研究结果提供了一株裂解能力强且海淡水均可应用的蛭弧菌菌株,查明了其生物学特性及影响其蛭质体密度的因素,为蛭弧菌的高效利用奠定了基础。[Background] Bdellovibrio-and-like organisms(BALOs) can lyse pathogenic bacteria in aquaculture, so BALOs have enormous potential value in application. However, in practical applications, the inconsistency between BALOs’ growth conditions and the environment results in poor or even no effect. Therefore, it is critical to obtain BALOs with a broad range of adaptation. [Objective] The aim of this study was to screen out a euryhaline BALO for extensive application and increase its bdelloplast densities for better preservation. [Methods] A euryhaline BALO was isolate from a shallow bay with the host bacterium Bacillus subtilis. Molecular biological identification were carried out, and the biological characteristics as well as lysis properties of BDN-1 were studied. In addition, the factors affecting its bdelloplast densities such as monosodium glutamate, Ca2+ and Mg2+, indole were researched. [Results] A strain of euryhaline BALOs(named BDN-1) was isolated and identified. The optimal range of temperature, salinity and pH for BDN-1 were 20-30 °C, 0.5%-3.0%, 6.0-8.5. Lysis experiments on 30 tested strains showed that the lytic rate of BDN-1 was 86.7%, and for the 16 tested vibrios, the lytic rate was 87.5%. The monosodium glutamate, indole, Ca2+ and Mg2+ have effects on the bdelloplast densities of BDN-1. [Conclusion] This study provided a strain of BALOs with strong lysis ability which can be applied to sea and fresh water. Besides, the biological characteristics and factors of bdelloplast densities were identified, which laid the foundation for the efficient application of BALOs.

关 键 词：蛭弧菌 广盐性 生物学特性 裂解能力 蛭质体密度 

分 类 号：S94[农业科学—水产养殖]