石家庄汉族人群血小板HPA-18～21基因多态性研究  

Polymorphism of platelet HPA-18-21 gene in Han population of Shijiazhuang

作　　者：胡光磊[1] 苏蔓[1] 张泓楠 王振雷[1] 何路军[1] HU Guanglei;SU Man;ZHANG Hongnan;WANG Zhenlei;HE Lujun(Hebei Province Blood Center,Shijiazhuang,050071,China)

机构地区：[1]河北省血液中心配型科

出　　处：《临床血液学杂志（输血与检验）》2019年第5期747-750,共4页Journal of Clinical Hematology(Blood Transfusion & Laboratory Medicine)

基　　金：河北省医学适用技术跟踪项目(No:G2018088)

摘　　要：目的:了解石家庄汉族献血人群血小板抗原HPA-18~21基因多态性。方法:采用PCR-SSP法对231例石家庄地区汉族无偿献血者进行HPA-18~21基因分型,并采用DNA测序验证。结果:231例石家庄汉族献血者中,全部样本均为HPA-18a/-18a、-19a/-19a、-20a/-20a纯合子,等位基因频率为100%;所有样本中227例为HPA-21a/-21a纯合子,4例为HPA-21a/-21b杂合子,并对杂合子进行了DNA测序证实,HPA-21a和HPA-21b的等位基因频率分别为99.13%和0.87%,与Hardy-Weinberg平衡吻合;在随机输血中HPA-21抗原不配合率为1.71%。结论:获得了石家庄汉族献血人群HPA-18~21基因多态性数据,为完善本地现有的HPA供者数据库、建立本地化已知HPA-18~21型别供者库提供了数据资料。Objective:To analysis the polymorphism of human platelet antigen HPA-18-21 gene in Han blood donors in Shijiazhuang.Method:Using SSP method to perform HPA-18-21 genotyping on 231 Han voluntary blood donors in Shijiazhuang,and verified by DNA sequencing.Result:All of the 231 Shijiazhuang Han blood donors were homozygous for HPA-18a/-18a,-19a/-19a,and-20a/-20a with an allele frequency of 100%.In all samples,227 were HPA-21a/-21a homozygotes and 4 were HPA-21a/-21b heterozygotes,then the heterozygotes were confirmed by DNA-sequencing,gene frequencies of HPA-21a and HPA-21b were 99.13%and 0.87%respectively,which fit Hardy-Weinberg equilibrium.The chance of incompatibility in random platelet transfusion was 1.71%for HPA-21.Conclusion:Gene frequencies of HPA-18-21 in Han blood donors in Shijiazhuang were obtained,which provided to improve the existing HPA database and establish a localized known HPA-18-21 donor database.

关 键 词：人类血小板抗原-18~21 基因频率 SSP法 DNA测序 

分 类 号：Q343.1[生物学—遗传学]