武汉地区健康成人网织红细胞相关参数生物参考区间调查  

Investigation on biological reference interval of reticulocyte parameters in healthy adult from Wuhan

作　　者：何小霞 李果 张洪波[1] 张驰 HE Xiaoxia;LI Guo;ZHANG Hongbo;ZHANG Chi(Department of Clinical Laboratory,Tongji Hospital,Tongji Medical College,Huazhong Universityof Science and Technology,Wuhan,430030,China)

机构地区：[1]华中科技大学同济医学院附属同济医院检验科

出　　处：《临床血液学杂志（输血与检验）》2019年第5期769-772,776,共5页Journal of Clinical Hematology(Blood Transfusion & Laboratory Medicine)

摘　　要：目的:用XN-9000建立武汉地区健康成人的网织红细胞相关参数的生物参考区间,为临床对贫血相关性疾病诊疗提供更为可靠的依据。方法:根据WS/T 402-2012文件的要求,选取华中科技大学同济医学院附属同济医院健康体检成人的静脉血437例,通过XN-9000仪器对[网织红细胞相关参数网织红细胞计数绝对值(RET#)、网织红细胞计数相对值(RET%)、高荧光网织红细胞比率(HFR%)、中荧光网织红细胞比率(MFR%)、低荧光网织红细胞比率(LFR%)和网织红细胞血红蛋白含量(Ret-He)]进行检测并调查其参考区间。结果:各项参数不同性别比较,差异有统计学意义(P<0.05);而不同年龄组比较,差异无统计学意义(P>0.05)。健康成年男性网织红参考区间RET#(0.038~0.100)×10^12/L、RET%0.63%~1.69%、HFR 0~1.90%、MFR 2.90%~11.80%、LFR 86.90%~97.10%、Ret-He 30.47~34.27pg;健康成年女性网织红参考区间RET#为(0.029~0.087)×10^12/L、RET%0.55%~1.59%、HFR 0~2.11%、MFR 1.60%~11.87%、LFR 85.55%~98.30%,Ret-He 29.37~33.18pg。结论:不同地区人群因生活环境、生活习惯的不同,其网织红细胞相关参数生物参考区间存在差异,而初步调查武汉地区的参考区间,对本地区的贫血相关性疾病的诊断与治疗都有辅助意义。Objective:To establish the reference interval of related parameters of reticulocyte in healthy adults from Wuhan using XN-9000,so as to provide a more reliable basis for clinical diagnosis and treatment of anaemia related diseases.Method:According to the document of WS/T 402-2012,437 cases of venous blood from healthy adults who underwent physical examination in Tongji Hospital were selected,and related parameters of reticulocytes were determined by the XN-9000(RET#,RET%,HFR%,MFR%,LFR%and RET-He)for analysing the reference range.Result:There were statistically significant differences in gender(P<0.05).However,there was no significant difference among ages(P>0.05).The reference intervals of RET#was(0.038-0.100)×10^12/L,RET%was 0.63%-1.69%,HFR was 0-1.90%,MFR was 2.90%-11.80%,LFR was 86.90%-97.10%,and Ret-He was 30.47-34.27 pg in healthy adult male.The reference intervals of RET#was 0.029-0.087×10^12/L,RET%was 0.55%-1.59%,HFR was 0-2.11%,MFR was 1.60%-11.87%,LFR was 85.55%-8.30%,and Ret-He was 29.37-33.18 pg in healthy adult female.Conclusion:Due to the differences in the living environment and habits,the reference ranges of reticulocytes are different in different regions.Preliminary investigation on the reference interval in Wuhan,it is helpful to the diagnosis and treatment of anaemia related diseases for local physician.

关 键 词：网织红细胞 荧光强度网织红细胞 网织红细胞血红蛋白含量 XN-9000 生物参考区间 

分 类 号：R555[医药卫生—血液循环系统疾病]