高效液相色谱法测定防晒类化妆品中20种防晒剂的含量  

Determination of 20 Sunscreen Agents in Sunscreen Cosmetics by High Performance Liquid Chromatography

作　　者：孙晶 曹玲 冯有龙 王伟国 杭太俊[2] SUN Jing;CAO Ling;FENG Youlong;WANG Weiguo;HANG Taijun(Jiangsu Institute for Food and Drug Control,Nanjing 210019,China;College of Pharmacy,China Pharmaceutical University,Nanjing 210008,China)

机构地区：[1]江苏省食品药品监督检验研究院,南京210019 [2]中国药科大学药学院,南京210008

出　　处：《理化检验（化学分册）》2019年第10期1186-1193,共8页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

摘　　要：称取混合均匀的防晒类化妆品样品0.200 0g于50mL具塞比色管中,用甲醇-四氢呋喃-水-高氯酸(350+550+100+0.067)溶液溶解,超声提取30min,移取2.0mL上述溶液,用甲醇-四氢呋喃-水-高氯酸(250+450+300+0.2)溶液稀释至10.0mL,经0.45μm滤膜过滤,弃去初虑液至少2mL,取续虑液作为待测液。采用高效液相色谱法测定其中20种防晒剂的含量,利用二极管阵列检测器检测。结果表明:20种防晒剂的质量浓度在一定范围内与其对应的峰面积之间呈线性关系,检出限(3S/N)在0.006 0~0.28mg·L^-1之间。以阴性防晒剂化妆品样品为基质,按照标准加入法进行回收试验,回收率在93.2%~107%之间,测定值的相对标准偏差(n=6)在0.10%~1.7%之间。0.200 0 g of sunscreen cosmetics were taken and dissolved with methanol-tetrahydrofuran-waterperchloric acid(350+550+100+0.067)solution in the 50 mL of colorimetric tube with plug.And then extracted ultrasonically for 30 min,remove 2.0 mL of the above solution and diluted it with methanol-tetrahydrofuran-waterperchloric acid(250+450+300+0.2)solution to 10.0 mL.The mixture was filtered through a filtering membrane of 0.45μm,at least 2 mL of the primary filtrate was discarded and the secondary filtrate was taken as the liquid to be tested.The contents of 20 sunscreens were determined by high performance liquid chromatography and detected by diode array detector.As shown by the results,linear relationships between values of peak area and mass concentration of the 20 sunscreens were found in the definite ranges,with detection limits(3 S/N)in the range of0.006 0-0.28 mg·L^-1.Test for recovery by standard addition method was made on the base of negative sunscreen cosmetic sample,giving results in the range of 93.2%-107%,and RSDs(n=6)ranged from 0.10%to 1.7%.

关 键 词：高效液相色谱法 防晒剂 防晒类化妆品 

分 类 号：O657.7[理学—分析化学]