氧化钆纳米粒子对A549肺癌细胞的辐射增敏效应研究  

Radiosensitizing Effect of Gadolinium Oxide Nanoparticles on Human Non-small Cell Lung Cancer A549 Cells

作　　者：李翡翡[1,2] 李子厚 金晓东 吴爱国[3] 陈卫强[1] 李强[1] LI Feifei;LI Zihou;JIN Xiaodong;WU Aiguo;CHEN Weiqiang;LI Qiang(Institute of Modern Physics,Chinese Academy of Sciences,Lanzhou 730000,China,Key Laboratory of Heavy Ion Radiation Biology and Medicine of Chinese Academy of Sciences,Key Laboratory of Basic Research on Heavy Ion Radiation Application in Medicine,Gansu Province,Lanzhou 730000,China;School of Nuclear Science and Technology,University of Chinese Academy of Sciences,Beijing 100049,China;Key Laboratory of Magnetic Materials and Devices,Chinese Academy of Sciences,Division of Functional Materials and Nano Devices,Ningbo Institute of Materials Technology&Engineering,Chinese Academy of Sciences,Ningbo 315201,Zhejiang China)

机构地区：[1]中国科学院近代物理研究所,中国科学院重离子束辐射生物医学重点实验室,甘肃省重离子束辐射医学应用基础重点实验室,兰州730000 [2]中国科学院大学核科学与技术学院,北京100049 [3]中国科学院宁波材料技术与工程研究所,浙江宁波315201

出　　处：《原子核物理评论》2019年第3期373-378,共6页Nuclear Physics Review

基　　金：国家重点研发计划项目(2017YFC0107500);甘肃省重大科技专项项目(1602FKDA005);中国科学院重离子束辐射生物医学重点实验室开放基金(2016-01)~~

摘　　要：采用氧化钆纳米粒子(GON),研究钆基纳米粒子对X射线和碳离子束的辐射增敏效应。首先,通过透射电镜观察材料粒径,使用DLS检测材料的水合半径及Zeta电位,并用紫外吸收谱证实GON在培养基中稳定性较好;研究发现钆(Gd)浓度为10.0μg/mL的GON对30 keV/μm碳离子束辐照水溶液产生的羟自由基的增强系数为1.13;GON对A549肺癌细胞和正常MRC-5肺细胞没有明显的毒性,且在人肺癌A549细胞中的摄取量随共培养浓度的增加而增加,在10.0μg/mL共培养浓度下,细胞摄入Gd的量为0.73 pg/cell;进一步采用克隆存活实验证明,GON的加入对X射线和碳离子辐照A549细胞所产生的损伤具有明显的增强,在10%的细胞存活水平下,GON对A549细胞在X射线及碳离子辐照下的辐射增敏分别达15.5%和10.1%。鉴于钆材料常被用于磁共振成像(MRI),所获得的GON有望作为X射线和碳离子的诊疗一体化材料。In this paper,the radiosensitizing effects of gadolinium based nanoparticles to X-ray and carbon ion beams were studied by using gadolinium oxide nanoparticles(GON).Firstly,the particle size of GON was measured by transmission electron microscopy,the hydration radius and Zeta potential of GON were measured by dynamic light scattering(DLS),and the stability of gadolinium nanoparticles in the medium was confirmed by ultraviolet absorption spectrum.It was found that GON with 10.0μg/mL gadolinium concentration enhanced the hydroxyl radical productivity by 1.13 times in aqueous solution under 30 keV/μm carbon ion beam irradiation.In addition,GON had no obvious toxicity to A549 lung cancer cells and normal MRC-5 lung cells.The uptake of gadolinium in human lung cancer A549 cells enhanced with the increase of co-culture GON concentration,and the uptake of gadolinium was 0.73 pg/cell when the co-culture concentration of GON was 10.0μg/mL.Most importantly,the radiosensitizing effect of GON on A549 cells irradiated with X-rays and carbon ions was assessed with the clonogenic survival assay.Our experimental results indicated that obviously radiosensitizing effect of GON on A549 cells was observed.The radiation enhancement ratio of GON on A549 cells exposed to X-rays and carbon ions was 15.5%and 10.1%at 10%survival level.Since gadolinium has been widely used for magnetic resonance imaging(MRI),the obtained GON is expected to be an ideal material for the diagnosis and treatment of cancer using X-ray and carbon ions radiotherapy.

关 键 词：氧化钆纳米粒子 人肺癌A549细胞 辐射增敏效应 

分 类 号：Q691[生物学—生物物理学]