黄连素对高糖环境下成骨细胞MC3T3-E1的影响  

The effect of berberine on osteoblast MC3T3-E1 cells under high glucose culture

作　　者：元建民 李璐[2] 余阳[3] 朱智敏[2] YUAN Jian-min;LI Lu;YU Yang;ZHU Zhi-min(College of Medicine Chengdu University,Chengdu 610075,Sichuan;West China School/Hospital of Stomatology of Sichuan University,Chengdu 610000,Sichuan;Chengdu University of Traditional Chinese Medicine,Chengdu 610075,Sichuan)

机构地区：[1]成都大学医学院,四川成都610075 [2]四川大学华西口腔医院(医学院),四川成都610000 [3]成都中医药大学,四川成都610075

出　　处：《中药与临床》2019年第2期23-26,共4页Pharmacy and Clinics of Chinese Materia Medica

摘　　要：目的:研究黄连素在高糖环境下对成骨细胞MC3T3-E1的影响作用。方法:建立体外细胞高糖培养环境,用黄连素进行干预,通过碱性磷酸酶试剂盒分别检测细胞内和细胞分泌的ALP水平;通过实时荧光定量PCR技术检测成骨细胞相关矿化基因RUNX2、ALP、OCN、COL-1的mRNA表达量。结果:高糖显著抑制了细胞的ALP活性和分泌;黄连素干预促进了成骨细胞ALP的活性和分泌,并可以显著提高成骨细胞相关矿化基因RUNX2、 ALP、 OCN、COL-1的mRNA表达量。结论:黄连素对高糖环境下的成骨细胞的ALP分泌和成骨相关基因的表达有显著促进作用,可逆转高糖导致的细胞成骨能力的降低。Objective: To explore the effect of berberine on MC3T3-E1 cells under high glucose culture. Method: The MC3T3-E1 cells were treated with berberine under high glucose culture. Alkaline Phosphatase Assay Kit was used to detect extraand intracellular secretion of ALP levels in MC3T3-E1 cells. Expression levels of RUNX2, ALP, OCN and COL-1 mRNA were quantified by real-time PCR. Result: ALP activity of MC3T3-E1 cells was significantly inhibited under high glucose culture. However, berberine significantly promoted the activity and secretion of ALP in MC3T3-E1 cells. RUNX2, ALP, OCN, COL-1 gene expressions were also up-regulated by berberine. Conclusion: Berberine effectively promoted MC3T3-E1 cells ALP activity and osteogenic genes expression under high glucose culture, reversing the inhibitory effects of high glucose on cells osteogenic ability.

关 键 词：黄连素 高糖环境 成骨细胞 

分 类 号：R285.5[医药卫生—中药学]