Effect of compound Uncaria Antihypertensive Tablet on protein expression of CT-1/JAKSTAT pathway in rats  

作　　者：ZENG Yong 曾勇(1st Affili Hosp of Hunan Univ TCM)

机构地区：[1]1st Affili Hosp of Hunan Univ TCM

出　　处：《China Medical Abstracts(Internal Medicine)》2019年第3期137-137,共1页中国医学文摘（内科学分册（英文版）

摘　　要：Objective To explore the mechanism of reversing left ventricular hypertrophy in spontaneous hypertension rats(SHR)by the intervention of Compound Uncaria Antihypertensive Tablet from the point of view of interfering with the protein expression of cardiotrophin-1(CT-1)/JAK-STAT pathway in rats'cardiac myocardium.Methods Totally 50 SHR were divided into 5 groups,the high dose group(group H,129.6 mg/mL),the middle dose group(group M,86.4 mg/mL),the low dose group(group L,43.2 mg/mL),the benazepril group(group B,0.9 mg/mL),and the SHR model control group(group S)according to the random number table,10 in each group.10 Wistar rats were recruited in a normal control group(group W),then perfomed gastric lavage once a day,and continued the intervention for 24 weeks.The contents of partial myocardial CT-1 and protein expression level of myocardial JAK1/STAT3 were detected by immunohistochemistry and Western blot respectively.Results Compared with group W,the expression level of CT-1 and the protein expression level of JAK1 and STAT3 in myocardial tissue were uniformly increased in group S(P<0.05).Compared with group S,expression levels of CT-1 in myocardial tissue decreased(P<0.05),while protein expression level of JAK1 and STAT3 decreased in group B,H,M and L(P<0.05).There was no statistically significant difference in the expression of CT-1 among the intervention groups(P>0.05).The protein expression levels of dose-dependent JAK1 and STAT3 increased in group H,M and L,and the difference was statistically significant(P<0.05).Compared with group B,the expression level of JAK1 and STAT3 decreased,the difference was statistically significant(P<0.05).Conclusion Compound Uncaria Antihypertensive Tablet had a certain inhibitory effect on the protein expression of CT-1/JAK-STAT pathway protein,and its possible mechanism to reverse left ventricular hypertrophy is to reduce expression level of cardiac partial CT-1 and inhibit the JAK-STAT pathway,and then give full scope to reverse the left ventricular hypertrophy.

关 键 词：HYPERTENSIVE STAT3 ventricular 

分 类 号：R54[医药卫生—心血管疾病]