DNA倍体分析在宫颈癌早期筛查中的应用价值  

Application value of DNA ploidy analysis in early screening of cervical cancer

作　　者：田磊 陈逢振[2] TIAN Lei;CHEN Feng-zhen(Obstetrics and Gynecology Department,Affiliated Hospital of Nankai University(Tianjin Fourth Hospital),Tianjin 300222,China;Second Hospital of Tianjin Medical University)

机构地区：[1]南开大学附属医院(天津市第四医院)妇产科,天津300222 [2]天津医科大学第二医院

出　　处：《中国城乡企业卫生》2019年第11期33-35,共3页Chinese Journal of Urban and Rural Enterprise Hygiene

摘　　要：目的探讨DNA倍体定量分析在诊断宫颈早期病变中的应用价值。方法选择2018年7-12月在南开大学附属医院(天津市第四医院)妇科门诊行阴道镜下宫颈活检300例患者,术前均行液基细胞学检查(TCT)和DNA倍体定量分析。以组织学病理结果为诊断金标准,评价两种方法在宫颈早期病变筛查中的作用。结果 300例患者病理显示:炎性34例,CIN Ⅰ 45例,CIN Ⅱ~Ⅲ 211例,宫颈癌10例。122例TCT阳性者中有103例病理结果阳性,178例阴性者中有118例病理结果阳性,其中3例TCT结果阴性,而病理学组织结果确诊为宫颈癌。185例DNA倍体定量分析阳性者中有158例病理结果阳性,115例阴性者中有63例病理结果阳性。DNA倍体定量分析对组织病理学的诊断灵敏度明显高于TCT(71.49%vs. 46.61%),差异有统计学意义(P<0.05),且无宫颈癌漏诊病例。结论 DNA倍体定量分析用于宫颈早期病变筛查,可明显提高敏感性,降低漏诊率,有利于早期诊治。Objective To explore the application value of DNA ploidy quantitative analysis in the diagnosis of early cervical lesions. Methods From July to December 2018,three hundred patients underwent cervical biopsy under colposcope in the gynecological outpatient department of Affiliated Hospital of Nankai University(Tianjin Fourth Hospital)were selected. All patients underwent thinprep cytologic test(TCT)and DNA ploidy quantitative analysis before operation. To evaluate the role of the two methods in the screening of early cervical lesions, with histopathological results as the gold diagnostic standard. Results The pathological examination of the 300 patients revealed:inflammatory in 34 cases,CIN Ⅰ in 45 cases,CIN Ⅱ-Ⅲ in 211 cases,and cervical cancer in 10 cases. Among 122 TCT positive patients,103 cases had positive pathological results,and among 178 negative patients 118 cases had positive pathological results,the cervical cancer of 3 TCT negative cases were confirmed by pathology. Among the 185 cases with positive DNA ploidy,158 cases had positive pathological results,and among the 115 cases with negative pathological results,63 cases had positive pathological results. The sensitivity of DNA ploidy quantitative analysis for histopathological diagnosis was significantly higher than that of TCT(71.49% vs. 46.61%),the difference was statislically significant(P<0.05),and there was no case of missed diagnosis of cervical cancer.Conclusion DNA ploidy quantitative analysis could be used for early cervical lesions screening,and it could significantly improve the sensitivity,reduce the rate of missed diagnosis,conducive to early diagnosis and treatment.

关 键 词：液基细胞学 DNA倍体定量分析 宫颈病变 早期筛查 

分 类 号：R711.74[医药卫生—妇产科学]