茶枯饼提取物对骨髓来源巨噬细胞炎症反应的作用研究  

Effect of camellia cake extracts on inflammatory response of bone marrow-derived macrophages

作　　者：王斐 何伟亮 谢璐 曾招林[3] 陈水亲[2] 宋涛[2] 刘志平[2] WANG Fei;HE Wei-liang;XIE Lu;ZENG Zhao-lin;CHEN Shui-qin;SONG Tao;LIU Zhi-ping(Gannan Medical University,Ganzhou,Jiangxi 341000;Gannan Medical University,School of Basic Medicine,Ganzhou,Jiangxi 341000;Gannan Medical University,The First Affiliated Hospital,Ganzhou,Jiangxi 341000)

机构地区：[1]赣南医学院,江西赣州341000 [2]赣南医学院基础医学院,江西赣州341000 [3]赣南医学院第一附属医院,江西赣州341000

出　　处：《赣南医学院学报》2019年第10期979-983,共5页JOURNAL OF GANNAN MEDICAL UNIVERSITY

基　　金：国家自然科学基金项目(31560260);江西省教育厅科研项目(GJJ170872,GJJ180802);赣南医学院人才启动基金项目(QD201404);赣南医学院校级油茶专项课题(YC201501);赣南油茶产业开发协同创新中心开放基金项目(YK201603,YK201615);赣南医学院2018年度研究生创新课题(YC2018-X003)

摘　　要：目的:研究茶枯饼提取物(CCEs)对脂多糖(LPS)诱导的小鼠骨髓来源巨噬细胞(BMDM)细胞炎症反应的作用。方法:培养BMDM后,给予不同浓度的CCEs处理12 h,再利用LPS刺激BMDM细胞,最后用PCR方法测定相关炎性细胞因子的表达水平。结果:LPS刺激BMDM 2~4 h引起炎症细胞因子表达的差异有统计学意义(P<0.05)。与LPS组对照,各浓度的CCEs处理的BMDM细胞在LPS刺激后相关经典炎症细胞因子,主要有白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)、角质细胞趋化因子(KC)和单核细胞趋化蛋白1(MCP-1)的表达水平差异无统计学意义(P>0.05)。结论:CCEs在目前LPS刺激的BMDM细胞的条件下没有显示抗炎症效应。Objective:To study the effect of Camellia cake extracts(CCEs)on the inflammatory response of mouse bone marrow-derived macrophages(BMDM)stimulated by lipopolysaccharide(LPS).Methods:BMDM cells were treated by CCEs at different concentrations for 12 hours,then stimulated by LPS.After that,the expression levels of relevant inflammatory cytokines were determined by PCR.Results:LPS stimulation of BMDM for 2-4 hours resulted in a significant increase(P<0.05)in the expression of inflammatory cytokines.Compared to LPS group,there was no significant difference(P>0.05)in the expression of inflammatory cytokines,including IL-6,TNF-α,KC,and MCP-1,in BMDM cells treated with CCEs at different concentrations after LPS stimulation.Conclusions:CCEs have no anti-inflammatory effect on BMDM cells stimulated by LPS under current experimental conditions.

关 键 词：茶枯饼提取物 巨噬细胞 炎症细胞因子 

分 类 号：R285.5[医药卫生—中药学]