不同质量浓度异烟肼对体外培养乳鼠成骨细胞的毒性作用  

Toxic effects of different-concentration isoniazid on newborn rat osteoblasts in vitro

作　　者：陈强 卓鸿武 夏天[2] 叶哲伟[2] Chen Qiang;Zhuo Hongwu;Xia Tian;Ye Zhewei(Fuzhou Second Hospital Affiliated to Xiamen University,Fuzhou 350007,Fujian Province,China;Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,Hubei Province,China)

机构地区：[1]厦门大学附属福州第二医院,福建省福州市350007 [2]华中科技大学同济医学院附属协和医院,湖北省武汉市430022

出　　处：《中国组织工程研究》2020年第8期1162-1167,共6页Chinese Journal of Tissue Engineering Research

基　　金：湖北省自然科学基金（0202040401），项目负责人：夏天~~

摘　　要：背景:异烟肼作为抗结核的一线药物,对细胞内外结核菌都具有极强的杀菌效果,但目前还没有研究报道异烟肼对成骨细胞的毒性作用。目的:探讨不同质量浓度异烟肼对新生SD大鼠成骨细胞增殖、分化的影响。方法:将不同质量浓度(0,10,20,30,40,50,60,100 mg/L)异烟肼分别加入新生SD大鼠第3代成骨细胞中,采用CCK-8法、碱性磷酸酶活性试剂盒和免疫荧光染色法检测异烟肼对成骨细胞增殖、分化的影响。结果与结论:与对照组比较,异烟肼质量浓度在30 mg/L时,成骨细胞增殖开始受到抑制;随着异烟肼质量浓度增高,碱性磷酸酶活性、Ⅰ型胶原表达明显降低。结果表明,异烟肼质量浓度过高对成骨细胞增殖和分化具有抑制作用。BACKGROUND:As a first-line antituberculosis drug,isoniazide exerts strong sterilization effect on the Tubercle bacillus inside and outside the cells.But the toxic effect of isoniazide on osteoblasts is never reported.OBJECTIVE:To investigate the effect of different concentrations of isoniazide on the proliferation and differentiation of osteoblasts from newborn Sprague-Dawley rats.METHODS:Isoniazide treatment at 0,10,20,30,40,50,60,and 100 mg/L was applied on passage 3 osteoblasts from newborn Sprague-Dawley rats.Then,osteoblast proliferation and differentiation was detected using cell counting kit-8 assay,alkaline phosphatase activity kit and immunohistochemical staining.RESULTS AND CONCLUSION:Compared with the control group,the proliferation of osteoblasts was significantly inhibited when the isoniazide concentration was 30 mg/L.In addition,increasing isoniazide concentrations inhibited alkaline phosphatase activity and type I collagen expression.To conclude,isoniazide at exorbitant concentration exerts adverse effects on the proliferation and differentiation of osteoblasts.

关 键 词：异烟肼 成骨细胞 碱性磷酸酶 Ⅰ型胶原 细胞增殖 细胞分化 

分 类 号：R459.9[医药卫生—治疗学] R318[医药卫生—临床医学]