腰痹舒干预腰椎间盘退变模型兔髓核组织水通道蛋白1、水通道蛋白3的表达  

Effect of Yaobishu on protein expression of aquaporin 1 and aquaporin 3 in nucleus pulposus of the degenerated lumbar intervertebral disc in a rabbit

作　　者：陈铁柱[1] 王宁科 李晓声[1] Chen Tiezhu;Wang Ningke;Li Xiaosheng(Hunan Provincial People’s Hospital,Changsha 410002,Hunan Province,China)

机构地区：[1]湖南省人民医院

出　　处：《中国组织工程研究》2020年第8期1168-1173,共6页Chinese Journal of Tissue Engineering Research

基　　金：湖南省科技创新计划项目（2018SK50717），项目负责人：陈铁柱~~

摘　　要：背景:椎间盘的老化和病变与椎间盘缺乏血液供应营养密切相关。水通道蛋白在椎间盘营养供应中发挥重要作用,但具体作用机制尚未完全明确。目的:基于水通道蛋白1、水通道蛋白3表达变化研究腰痹舒作用于兔椎间盘退变的机制。方法:取36只新西兰大白兔,将生理盐水注入L 4/5、L 5/6椎间盘制备腰椎间盘突出症模型,然后随机分成模型组、低剂量腰痹舒组、高剂量腰痹舒组,术后8周,模型组灌胃生理盐水5 mL/(kg d),低剂量腰痹舒组灌胃腰痹舒5 mL/(kg d),高剂量腰痹舒组灌胃腰痹舒10 mL/(kg d),2次/d,共21 d。治疗后6周,取L 4/5、L 5/6椎间盘分别进行解剖大体观察、组织切片苏木精-伊红染色、RT-PCR、Western blot检测,观察3组髓核组织外观形态、病理形态以及水通道蛋白1、水通道蛋白3 mRNA和蛋白表达。结果与结论:①各组均可见纤维环破坏,出现异常软骨组织,髓核减少,高剂量腰痹舒组病变程度轻于低剂量腰痹舒组,模型组破坏最明显;②治疗6周,高剂量腰痹舒组、低剂量腰痹舒组水通道蛋白1、水通道蛋白3 mRNA和蛋白表达量增高,与治疗前比较差异有显著性意义(P<0.05);模型组治疗前后比较差异无显著性意义(P>0.05);组间对比3组水通道蛋白1和水通道蛋白3 mRNA和蛋白表达,差异有显著性意义(P<0.05);③结果表明,腰痹舒可能是通过提高水通道蛋白1、水通道蛋白3的表达量,减轻兔椎间盘退变。BACKGROUND: The aging and lesions of the intervertebral disc are closely related to the lack of nutritional blood supply to the disc. Aquaporin plays an important role in the nutritional supply to the intervertebral discs, but the specific mechanism has not been fully defined. OBJECTIVE: To study the mechanism of Yaobishu on degenerated intervertebral disc in rabbits based on the changes of aquaporin (AQP) 1 and AQP3 protein expression. METHODS: Thirty-six New Zealand white rabbits were randomly divided into model group, low-dose Yaobishu group and high-dose Yaobishu group. Animal models of lumbar intervertebral disc prolapse were prepared through an injection of normal saline into L4/5 and L5/6 segments. The model group was intragastrically given normal saline 5 mL/kg per day, the low-dose group was intragastrically given Yaobishu 5 mL/kg per day, and the high-dose group was intragastrically given Yaobishu 10 mL/kg per day, twice a day, for 21 days. After 6 weeks of treatment, the intervertebral discs were taken for anatomical and histological observation using hematoxylin-eosin staining. Expression of AQP1 and AQP3 in the nucleus pulposus at protein and mRNA levels was quantified by RT-PCR and western blot assay. RESULTS AND CONCLUSION: In all the three groups, the annulus fibrosus was destroyed, abnormal cartilage tissue appeared, and the nucleus pulposus was reduced in number. Severest degeneration of the intervertebral disc was found in the model group, followed by the low-dose Yaobishu and high-dose Yaobishu groups in turn. The expression of AQP1 and AQP3 mRNA and protein in the high-dose Yaobishu group and low-dose Yaobishu group increased significantly after 6 weeks of treatment (P < 0.05), while the expression in the model group showed no significant difference before and after treatment (P > 0.05). There were significant differences in the expression of AQP1 and AQP3 mRNA and protein among the three groups (P < 0.05). Therefore, Yaobishu may alleviate the degeneration of the rabbit intervertebral disc b

关 键 词：腰痹舒 腰椎间盘退变 水通道蛋白 水通道蛋白1 水通道蛋白3 

分 类 号：R459.9[医药卫生—治疗学] R318[医药卫生—临床医学]