MiR-21可调控牙周炎破骨细胞的增殖  

MicroRNA-21 regulates proliferation and differentiation of osteoclasts in periodontitis

作　　者：许诺[1] 曹蓁 李晓杰[2] 史春[2] Xu Nuo;Cao Zhen;Li Xiaojie;Shi Chun(Zhongshan College of Dalian Medical University,Dalian 116000,Liaoning Province,China;School of Stomatology,Dalian Medical University,Dalian 116000,Liaoning Province,China)

机构地区：[1]大连医科大学中山学院,辽宁省大连市116000 [2]大连医科大学口腔医学院,辽宁省大连市116000

出　　处：《中国组织工程研究》2020年第8期1225-1230,共6页Chinese Journal of Tissue Engineering Research

基　　金：辽宁省自然科学基金（20180550563），项目负责人：史春~~

摘　　要：背景:miR-21是破骨细胞生成的调节剂和破骨细胞分化的启动子,但是其在牙周炎中的作用尚不清楚。目的:探讨miR-21在牙周炎骨破坏中的作用。方法:Real-time PCR法检测并分析牙周炎样本和正常牙周膜组织中miR-21的表达差异;利用脂质体转染法,以miR-21 mimics(上调miR-21)或miR-21 inhibitor(下调miR-21)转染破骨细胞,Real-time PCR法检测miR-21及骨破坏标志因子TRAP与CTSK的表达变化;CCK-8检测miR-21对破骨细胞的增殖作用。结果与结论:①miR-21在牙周炎样本中表达增高;②miR-21 mimics转染到破骨细胞后,miR-21、TRAP与CTSK mRNA的表达升高;miR-21 inhibitor转染到破骨细胞后,miR-21、TRAP与CTSK mRNA的表达下降;③miR-21 mimics转染到破骨细胞后,促进破骨细胞增殖;miR-21 inhibitor转染到破骨细胞后,抑制破骨细胞增殖;④结果表明,miR-21可以促进破骨细胞的增殖,结合miR-21可以促进骨破坏标志因子的表达,说明miR-21可以作为治疗牙周炎骨破坏的重要靶点。BACKGROUND: MicroRNA-21 (miR-21) is a regulator of osteoclastogenesis and a promoter of osteoclast differentiation, but its role in periodontitis remains unclear. OBJECTIVE: To investigate whether miR-21 is involved in bone destruction in periodontitis. METHODS: Real-time PCR was used to detect and analyze the differential expression of miR-21 in periodontitis samples. Using liposome transfection method, miR-21 mimics (up-regulating miR-21) or miR-21 inhibitor (down-regulating miR-21) was used to transfect osteoclasts. Expressions of miR-21 and bone destruction markers TRAP and CTSK were detected by real-time PCR. Cell counting kit-8 was used to detect the miR-21 effect on osteoclast proliferation. RESULTS AND CONCLUSION: (1) MiR-21 expression increased in periodontitis samples. (2) When miR-21 mimics was transfected into osteoclasts, miR-21, TRAP and CTSK mRNA expression increased;when miR-21 inhibitor was transfected into osteoclasts, miR-21, TRAP and CTSK mRNA expression decreased. (3) Transfection with miR-21 mimics promoted the proliferation of osteoclasts, while transfection with miR-21 inhibitor inhibited the proliferation of osteoclasts. To conclude, miR-21 can be used as an important target for the treatment of periodontitis.

关 键 词：牙周炎 RAW264.7细胞 破骨细胞 MIR-21 骨破坏标志因子 辽宁省自然科学基金 

分 类 号：R459.9[医药卫生—治疗学] R318[医药卫生—临床医学]