持续性内切纤维素酶高效催化的研究进展  

Advances in High Efficiency Catalysis of Persistent Endoglucanase

作　　者：陶敏 王振兴 陈晓艺[1] 李宪臻[1] TAO Min;WANG Zhen-xing;CHEN Xiao-yi;LI Xian-zhen(Schl.of Bio-Engineering,Dalian Polytechnic Uni.,Dalian,116034)

机构地区：[1]大连工业大学生物工程学院

出　　处：《微生物学杂志》2019年第5期98-104,共7页Journal of Microbiology

基　　金：国家自然科学基金项目(31801469);辽宁省博士启动基金项目(201601273);辽宁省教育厅A类项目(2017J029)

摘　　要：持续性内切纤维素酶(Processive endoglucanase)是一类新发现的双功能纤维素水解酶,既符合内切酶的作用特征,又具有外切酶的持续催化能力,可高效降解纤维素生成小分子寡糖。这类酶通常具有模块化结构,碳水化合物结合模块(CBM)对酶的持续催化活性及底物结合能力表现出不同的影响。综述了该领域相关研究的最新进展,分析了持续性内切酶潜在的研究方向及工业化应用的前景。Persistent endoglucanases(EG) are kinds of newly characterized bifunctional cellulases, with both properties of endoglucanase and exoglucanase. They could efficiently hydrolyze cellulose into low molecular oligosaccharides such as cellulose, cellotriose or cellotetraose. Carbohydrate-binding modules(CBM) are common in the structure of these enzymes, while their effects on the persistent catalytic activity and substrate-binding capacity of the enzymes are quite different. This article summarized the advances in high efficiency catalysis of persistent endoglucanase, and proposes the potential research directions and industrial applications of persistent endoglucanase.

关 键 词：持续性内切纤维素酶 酶活性表征 模块化组合 活性中心架构 

分 类 号：Q556.2[生物学—生物化学] Q71