犊牛肺炎多杀性巴氏杆菌荚膜血清型和基因型的研究  

ANALYSIS OF SEROTYPES AND GENOTYPES OF PASTEURELLA MULTOCIDA ISOLATES FROM PNEUMONIC CALVES

作　　者：梅朱园 邬琴[1] 柴迎锦 顾晓晓 陶乔孝慈 钟发刚[2] 韩猛立[2] 黄新[2] 周霞[1] 张星星[2] MEI Zhu-yuan;WU Qin;CHAI Ying-jin;GU Xiao-xiao;TAOQIAO Xiao-ci;ZHONG Fa-gang;HAN Meng-li;HUANG Xin;ZHOU Xia;ZHANG Xing-xing(College of Animal Science and Technology,Shihezi University,Shihezi,Xinjiang 832003,China;State Key Laboratory for Sheep Genetic Improvement and Healthy Production,Xinjiang Academy of Agricultural and Reclamation Science,Shihezi,Xinjiang 832000,China)

机构地区：[1]石河子大学动物科技学院,石河子832003 [2]新疆农垦科学院省部共建绵羊遗传改良与健康养殖国家重点实验室,石河子832000

出　　处：《中国动物传染病学报》2019年第5期44-49,共6页Chinese Journal of Animal Infectious Diseases

基　　金：2017年国家级创新创业训练计划项目(2017107590);省部共建绵羊遗传改良与健康养殖国家重点实验室优秀中青年人才培养引导计划专项项目(SKLSGIHP2016A02,SKLSGIHP2017A03);兵团重大科技攻关项目(2014AA001-3);兵团现代农业科技攻关与成果转化计划项目(2015AC011);兵团师域发展创新支持计划项目(2017BA038)

摘　　要：从石河子及附近地区规模化牛场死于肺炎的犊牛肺脏中共分离8株多杀性巴氏杆菌(Pasteurella multocida,Pm),为了确定分离菌株的血清型和基因型,分别进行了多重荚膜特异基因PCR检测、脂多糖多重PCR(LPS-m PCR)分型和多位点序列分型(MLST)鉴定和分析。结果显示8株分离菌中,7株为荚膜A型,1株为荚膜B型;LPS-m PCR结果显示其中7株菌为L2,1株为L3基因型;MLST技术将8株细菌中的7株分为ST1型,1株分为ST44型,且两者亲缘关系较近。结果表明8株Pm以荚膜血清A型为主,根据LPS基因特异性将其分为2个基因型,基于管家基因的MLST将8株Pm分为两种ST型。In order to investigate the serotypes and genotypes of 8 Pasteurella multocida(Pm)isolates from the lungs of calves that died of pneumonia on large-scale farms in Shihezi and nearby areas in recent years,multiple capsular PCR,LPS-m PCR and multilocus sequence typing(MLST)were used to analyze and identify the kmt genes,capsule serotypes and genotypes.The results showed that 8 isolates were divided into A and B capsular serotypes,of which 7 strains were type A and 1 strain was type B.These 8 isolates were further categorized into LPS genotypes L2 and L3 and MLST types ST-1 and ST-44.

关 键 词：多杀性巴氏杆菌 荚膜分型 脂多糖多重PCR 多位点序列分型 

分 类 号：S852.612[农业科学—基础兽医学]