恩诺沙星对17℃负压环境下猪精子保存效果的影响  

Effects of enrofloxacin on the preservation of pig sperm under a 17 ℃ negative pressure environment

作　　者：张兆博 郑丽云 谷圣臣 周琳琳 王双 孙天娇 李琦[1] 魏国生[1] 李井春[1] ZHANG Zhaobo;ZHENG Liyun;GU Shengchen;ZHOU Linlin;WANG Shuang;SUN Tianjiao;LI Qi;WEI Guosheng;LI Jingchun(College of Animal Science and Veterinary Medicine,Heilongjiang Bayi Agricultural University,Daqing 163319,China)

机构地区：[1]黑龙江八一农垦大学动物科技学院

出　　处：《黑龙江畜牧兽医》2019年第21期122-125,共4页Heilongjiang Animal Science And veterinary Medicine

基　　金：黑龙江省大学生创新创业训练计划项目(201810223049);黑龙江省自然科学基金面上项目(C2017053);黑龙江省农垦总局科研计划项目(HNK135-04-06);黑龙江八一农垦大学博士启动基金项目(XYB-2016-04);黑龙江八一农垦大学创新人才项目(CXRC2017004)

摘　　要：为研究猪精子在17℃负压环境下保存时不同浓度恩诺沙星对精子质量的影响,并探讨恩诺沙星的最适添加浓度,试验在Modena基础稀释液中添加不同浓度恩诺沙星并在17℃负压环境下保存猪精液,利用迈朗全自动精子质量检测仪等检查猪精子活率、路径速度、直线速度和畸形率。结果表明:恩诺沙星浓度为50μg/L时,精子活率、路径速度、直线速度和畸形率都显著高于其他浓度(P<0.05)。说明50μg/L的恩诺沙星有利于17℃负压环境下猪精液的保存。To study the effect of different concentrations of enrofloxacin on sperm quality under a 17℃negative pressure environment and to screen the optimum addition concentration of enrofloxacin,pig sperm was placed in Modena basic diluent with different concentration doses of enrofloxacin and was stored in a 17 negative pressure environment.Subsequently,sperm viability,path speed,straight line speed and deformity rate were examined using MAILANG automatic sperm quality detector.The results showed that sperm viability,path speed,straight line speed and deformity rate in the 50μg/L enrofloxacin group were significantly better than those in other groups(P<0.05),indicating that 50μg/L enrofloxacin was beneficial to the preservation of pig spermunder a 17℃negative pressure environment..

关 键 词：恩诺沙星 17℃ 负压 猪精子 精子质量 

分 类 号：S814.3[农业科学—畜牧学] S828[农业科学—畜牧兽医]