双歧杆菌MRS培养基优化研究  

Medium MRS Optimization of Bifidobacterium

作　　者：于亭 王振雷 Yu Ting;Wang Zhenlei(Liaoyuan Food Quality and Safety Testing Center,Liaoyuan 136200,China)

机构地区：[1]辽源市食品质量安全检测中心

出　　处：《现代食品》2019年第22期151-154,共4页Modern Food

摘　　要：本文以双歧杆菌为研究对象,研究双歧杆菌对培养基的营养需求,采用正交实验优化设计MRS培养基,优化培养基中碳源,氮源,增殖因子的用量。确定最适合该菌生长的培养基配比为:葡萄糖1%,复合氮源为2.5%,番茄汁7%。双歧杆菌在优化的MRS培养基中活菌数可达1.95×10^9 CFU·mL^-1,约为普通MRS培养基中菌数(5.92×10^8 CFU·mL^-1)的3.3倍。The Bifidobacterium was taken as the research object to study the nutritional requirements of the medium for Bifidobacteriume,orthogonal experiment was used to optimize the design of MRS medium,optimize the amount of carbon source,nitrogen source and proliferation factor in the medium.To determine the most suitable medium for the growth of the bacteria:glucose 1%,compound nitrogen source 2.5%,tomato juice 7%.In the optimized MRS medium,the viable count of Bifidobacterium could be as high as 1.95×10^9 CFU·mL^-1,which was about 3.3 times of the count of bacteria in the ordinary MRS medium(5.92×10^8 CFU·mL^-1).

关 键 词：双歧杆菌 培养基 改良MRS 

分 类 号：Q93[生物学—微生物学]