HLA-DP基因多态性与HCV患者抗病毒治疗免疫应答的相关性分析  

Correlation Analysis of HLA-DP Gene Polymorphisms with Immune Response to Antiviral Treatment for HCV Patients

作　　者：石菡[1] 李艳芳[1] 袁媛[1] 贾志华 纪风兵[1] SHI Han;LI Yanfang;YUAN Yuan;JIA Zhihua;JI Fengbing(Dept.of Infectious Diseases,the First Affiliated Hospital of Chengdu Medical College,Chengdu 610500,China)

机构地区：[1]成都医学院第一附属医院传染科

出　　处：《中国药房》2019年第22期3112-3117,共6页China Pharmacy

基　　金：四川省卫生厅科学研究项目（No.130382）

摘　　要：目的:研究HLA-DP基因多态性与丙型肝炎病毒(HCV)患者抗病毒治疗免疫应答的相关性。方法:选取2013年5月-2017年8月于我院就诊的HCV汉族患者106例,所有患者均接受为期48周的聚乙二醇化干扰素α(PEG IFNα)+利巴韦林(RBV)联合治疗,并于停药后接受为期24周的随访。记录其年龄、体质量、HCV-RNA基线水平等一般资料,并采用Taqman-MGB荧光探针实时定量聚合酶链反应法检测其HLA-DP基因rs3077、rs2395309位点的分型。根据治疗结局将患者分为持续病毒学应答组(SVR)和未持续病毒学应答组(N-SVR),采用Logistic回归模型对影响HCV患者抗病毒治疗免疫应答的相关因素(性别、年龄、体质量指数、HCV-RNA基线水平以及基因多态性)进行单因素和多因素分析。结果:106例患者中,rs3077位点CC、CT、TT型频率分别为40.6%、35.8%、23.6%,rs2395309位点GG、GA、AA型频率分别为50.0%、39.6%、10.4%,均符合Hardy-Weinberg遗传平衡(P>0.05)。SVR组患者80例,N-SVR组患者26例;SVR组患者的年龄、rs3077位点CT、TT型以及rs2395309位点GA、AA型比例均显著低于N-SVR组,rs3077位点CC型以及rs2395309位点GG型比例均显著高于N-SVR组(P<0.05);而两组患者的性别、体质量指数、HCV-RNA基线水平比较,差异均无统计学意义(P>0.05)。单因素、多因素分析结果显示,患者性别,体质量指数,HCV-RNA基线水平,rs3077位点CC型、rs2395309位点GG型与其抗病毒治疗免疫应答不显著相关(P>0.05),而年龄、rs3077位点CT和TT型、rs2395309位点GA和AA型与其抗病毒治疗免疫应答显著相关[比值比(OR)分别为1.135、1.766、1.283、1.218、1.103,95%置信区间(CI)分别为(1.017,1.267)、(1.007,3.100)、(1.038,1.585)、(1.011,1.467)、(1.038,1.172),P<0.05]。结论:年龄以及HLA-DP基因rs3077、rs2395309位点多态性与HCV汉族患者PEG IFNα+RBV抗病毒治疗免疫应答相关,其中低龄患者可能获得较高的抗病毒免疫应答率,而T、A突变等位基因携带�OBJECTIVE:To study the correlation of HLA-DP gene polymorphisms with the immune response to antiviral treatment for hepatitis C virus(HCV)patients.METHODS:A total of 106 HCV Han-nationality patients were collected from our hospital during May 2013 to Aug 2017.All patients received PEG IFNα+ribavirin(RBV)for 48 weeks,and then 24 week follow-up after drug withdrawal.Age,body weight and baseline level of HCV-RNA were recorded.The typing of rs3077 and rs2395309 site of HLA-DP gene were detected by RT-qPCR with Taqman-MGB fluorescent probe.According to treatment outcome,the patients were divided into two groups such as sustained viral response(SVR)group and no-sustained viral response(N-SVR)group.Univariate and multivariate analysis were performed for influential factors(gender,age,body weight index,HCV-RNA baseline level,gene polymorphisms)of immune response to antiviral treatment for HCV patients with Logistic regression model.RESULTS:Among 106 patients,the frequencies of CC,CT and TT genotype at rs3077 site were 40.6%,35.8% and 23.6%;those of GG,GA and AA genotype at rs2395309 site were 50.0%,39.6%,10.4%,respectively,which were in line with Hardy-Weinberg genetic balance(P>0.05).Totally 80 HCV patients were obtained in SVR group,and 26 HCV patients in N-SVR group.The patient’s age,the proportion of CT and TT genotype of rs3077 site and GA and AA genotype of rs2395309 site in SVR group were significantly lower than N-SVR group.The proportion of CC genotype at rs3077 site and GG genotype at rs2395309 site were significantly higher than N-SVR group(P<0.05).There was no statistical significance in gender,body weight index or HCV-RNA baseline level between 2 groups(P>0.05).Univariate and multivariate analysis showed that gender,body mass index,HCV-RNA baseline level,CC genotype at rs3077 site and GG genotype at rs2395309 site were not related to the immune response to antiviral treatment(P>0.05).Age,CT and TT genotype at rs3077 site,GA and AA genotype at rs2395309 site were associated with the immune response to anti

关 键 词：丙型肝炎病毒 汉族 HLA-DP基因 基因多态性 rs3077 rs2395309 抗病毒治疗免疫应答 相关性 

分 类 号：R511[医药卫生—内科学]