药用蕨类植物骨碎补(Drynaria fortune)根茎繁殖研究  

Study on the Rhizome Propagation of Drynaria fortune

作　　者：严福林 杜富强 涂俊 林红叶 孙庆文 魏升华 YAN Fulin;DU Fuqiang;TU Jun;LIN Hongye;SUN Qingwen;WEI Shenghua(Department of Pharmacy,Guizhou University of Traditional Chinese Medicine,Guiyang,Guizhou 550025)

机构地区：[1]贵州中医药大学药学院

出　　处：《北方园艺》2019年第21期95-101,共7页Northern Horticulture

基　　金：国家中医药管理局资助项目(2013-2015);国家自然科学基金喀斯特联合资助项目(U1812403-2-1);科技部国家重点研发计划资助项目(2018YFC1708101);贵州省科技计划资助项目(黔科合LH字[2016]7113号);贵州省科技计划资助项目(黔科合平台人才[2019]5658);贵阳中医学院大学生创新创业训练计划资助项目(贵中医大创合字[2017]17号);药用植物功效利用国家重点实验室开放课题资助项目(FAMP201701K);贵州省教育厅资助项目(黔教合KY字[2017]018);贵州省国内一流建设学科项目子课题(中药学)资助项目(GNYL[2017]008-7号)

摘　　要：以中药材骨碎补基源植物槲蕨为试材,应用多重比较与主成分分析方法考察不同基质、激素类型及其浓度与处理时间对骨碎补根茎生根率、生根数、根长、叶片数、新茎数、新茎长与根系效果指数的影响。结果表明:以砂石或砂石∶壤土=1∶1繁殖基质,可有效提高骨碎补插穗生根率和生根数;当基质为砂石∶壤土=1∶1时更有利于根系的生长,提高根系效果指数、促进骨碎补插穗生长。生长调节剂在一定浓度范围内对骨碎补生长具有一定的促进作用,但高于一定浓度呈抑制趋势。主成分分析结果表明,浓度为100 mg·L-1处理综合得分最高,处理1.5~3.0 h可显著提高骨碎补插穗根系效果指数,促进骨碎补根系发育与茎叶分化。该研究提供了促进骨碎补根茎根系发育与后期生长方法,可为骨碎补的人工繁殖提供参考。The Drynaria fortune was used as a test material.The effects of different substrates,hormone types and concentrations,and treatment time on rooting rate,rooting number,root length,leaf number,new stem number,new stem length and root effect index were investigated by multiple comparison and principal component analysis.The results showed that the rooting rate and rooting number of cuttings could be effectively increased by using sand or sand∶loam=1∶1 reproductive matrix for D.fortune.When the matrix was sand and loam=1∶1,it was more beneficial to the growth of root system,improve the root effect index and promote the growth of cuttings.Growth regulatorscould promote the growth of D.fortune in a certain concentration range,however,when their concentration was above a certain level,they tend to inhibit.The results of principal component analysis showed that the group with the concentration of 100 mg·L-1 had the highest comprehensive score,treatment of 1.5-3.0 hours could significantly improve the root effect index of cuttings of D.fortune,promote the development of root and stem and leaf differentiation.This study provided a method to promote the root development and later growth of D.fortune,which could provide a reference for the artificial reproduction of D.fortune.

关 键 词：骨碎补 繁殖 基质 生长调节剂 

分 类 号：S567.9[农业科学—中草药栽培]