利用LB膜技术的胶原覆层生物材料制备与表征  

Preparation and characterization of collagen-coated biomaterials by Langmuir-Blodgett films

作　　者：邱宁[1] QIU Ning(Qindao College,Qingdao Technological University,Qingdao 266106,China)

机构地区：[1]青岛理工大学琴岛学院

出　　处：《哈尔滨工程大学学报》2019年第10期1790-1794,共5页Journal of Harbin Engineering University

基　　金：国家自然科学基金青年科学基金项目(21306205);山东省高等学校科技计划项目(J17KB053)

摘　　要：针对羟基磷灰石材料强度低、韧度低的问题,本文提出了一种基于LB膜技术的胶原覆层生物材料制备方法。将胶原蛋白用碱性蛋白酶和木瓜蛋白酶酶解成分子量200~5000 Da的胶原蛋白肽,利用LB膜技术制备胶原蛋白肽单分子层,将其覆层至羟基磷灰石片上。采用原子力显微镜(AFM)对覆层的胶原蛋白肽膜进行表征,并对覆层材料进行了理化性能分析,最后通过MC3T3-E1细胞在覆层材料上的培养情况研究其生物性能。结果表明:AFM发现膜的表面平整,单层厚度约为3.1 nm,其覆层材料性质稳定,有良好的力学性能,杨氏模量最高可达6.21±0.18 MPa,MC3T3-E1细胞在覆层材料上生长良好。因此,可以利用LB膜技术制备新型胶原覆层材料,为研发新型医用生物覆层材料提供新思路。In order to solve the low strength and low toughness problem of hydroxyapatite materials,a method for preparation of collagen-coated biomaterials based on LB membrane technology is presented.Using alkaline protease and papain,the collagen protein was digested into a collagen peptide with a molecular weight of 200~5000 Da.Collagen peptide monolayers were prepared using LB membrane technology,and then coated onto hydroxyapatite tablets.The coated collagen peptide membrane was characterized by atomic force microscopy(AFM)to analyze the physical and chemical properties.Finally,the biological properties of MC3T3-E1 cells were studied by culturing on the coating material.It was found that the membrane surface was even and the monolayer thickness was about 3.1 nm.The coating material was stable in performance,having a better mechanical property,and the Young′s modulus was up to 6.21±0.18 MPa.The culture of MC3T3-E1 cells on the coating material was studied.It was found that the cells grew well on the coating material.Therefore,LB membrane technology can be used to prepare new collagen coating materials,which provides a new method for the development of new medical bio-coating materials.

关 键 词：胶原覆层 生物材料 医用生物覆层材料 LB膜 原子力显微镜 

分 类 号：Q819[生物学—生物工程]