乙基紫褪色分光光度法测定药物中的美司那  

Determination of mesna in drug by fading spectrophotometry with ethyl violet

作　　者：周于倩 江虹[1] ZHOU Yu-qian;JIANG Hong(College of Chemistry and Chemical Engineering,Collaborative Innovation Center for Green Development in Wuling Mountain Areas,Yangtze Normal University,Chongqing 408100,China)

机构地区：[1]长江师范学院化学化工学院武陵山片区绿色发展协同创新中心

出　　处：《化学研究与应用》2019年第11期1981-1984,共4页Chemical Research and Application

基　　金：重庆市教委科技基金项目(KJ1401226)资助;长江师范学院科技基金项目(2018CXX123)资助

摘　　要：建立了测定药物中美司那的褪色分光光度法。在酸性BR缓冲溶液中,美司那与乙基紫以静电引力作用生成具有2个较强负吸收峰的新物质,使溶液褪色,美司那的质量浓度在630 nm和492 nm处与生成的新物质的吸光强度的绝对值(|A|)有线性关系并服从朗伯-比尔定律,他们的线性范围为0.2~2.3 mg·L^-1,表观摩尔吸光系数(κ)分别为1.71×104 L/(mol·cm)(492 nm)和2.53×104 L/(mol·cm)(630 nm),检出限分别为0.18 mg·L^-1和0.14 mg·L^-1。用双波长叠加法测定时,其表观摩尔吸光系数(κ)达4.24×104 L/(mol·cm),约是单波长法的2倍,检出限为0.080 mg·L^-1。该法用于市售美司那药物中美司那含量的测定,结果满意。A new fading spectroscopy method for the determination of mesna in drugs was established.In an acidic BR buffer solution,mesna reacted with ethyl violet by electrostatic attraction to form a new substance with two strong negative absorption peaks,which led to the solution fade.At 630 nm and 492 nm,the mass concentration of mesna has a linear relationship with the absolute value of the absorbance(|A|)of the new substance,Lambert-Beer’s law was obeyed.Their linear range were 0.2 to 2.3 mg·L^-1,and the apparent molar absorptivity(κ)were 1.71×104 L/(mol·cm)(492 nm)and 2.53×104 L/(mol·cm)(630 nm)respectively,and the detection limits were 0.18 mg·L^-1 and 0.14 mg·L^-1,respectively.When the dual-wavelength spectrophotometry was used to determine the content of mesna,its apparent molar absorption coefficient(κ)can be achieved 4.24×104 L/(mol·cm),which was about twice that of the single-wavelength method,and the detection limit was 0.080 mg·L^-1.The method had been applied to the determination content of mesna in commercially available mesna drugs,and the satisfactory results.

关 键 词：美司那 乙基紫 药物 褪色分光光度法 

分 类 号：O657.32[理学—分析化学]