Alopecurone B逆转人乳腺癌细胞MCF-7阿霉素耐药株活性研究  

Reversal Effect and Mechanism of Alopecurone B on MCF-7 Adriamycin-Resistant Subline

作　　者：黄莉莉[1] 王欣[1] 李冰冰[1] 缪明星[2] HUANG Li-li;WANG Xin;LI Bing-bing;MIAO Ming-xing(Drum Tozver Hospital Affiliated to School of Medicine,Nanjing University,Nanjing,210008,China;School of pharmacy,China Pharmaceutical University,Nanjing,210009,China)

机构地区：[1]南京大学医学院附属鼓楼医院,江苏南京210008 [2]中国药科大学药学院,江苏南京210009

出　　处：《南京中医药大学学报》2019年第6期699-703,共5页Journal of Nanjing University of Traditional Chinese Medicine

基　　金：江苏省中医药管理局科技项目(YB2017046)

摘　　要：目的研究黄酮化合物Alopecurone B(APB)对人乳腺癌细胞阿霉素耐药株MCF-7/ADR多药耐药的逆转活性及逆转机制。方法人乳腺癌细胞阿霉素耐药株MCF-7/ADR维持在含有250 ng/mL阿霉素的培养基中,使用MTT法、qPCR、Western blot和流式细胞术研究APB对耐药株P-糖蛋白(P-gp)功能和表达的影响。结果 APB能逆转MCF-7/ADR细胞多药耐药,抑制P-gp的功能,下调P-gp基因和蛋白的表达。结论 APB具有强效逆转人乳腺癌细胞MCF-7/ADR多药耐药的效果,其机制涉及对P-gp的调控。OBJECTIVE To study the reversal effect and the mechanism of APB on adriamycin(ADR)-induced multidrug-resistant human breast cancer MCF-7/ADR cells. METHODS ADR-resistant MCF-7 cells were maintained in RPMI-1640 culture medium containing 10%FBS and 250 ng/mL ADR. MTT assay, qPCR, Western blot and flow cytometry were used to determine the inhibitory effect of APB on P-gp levels and function in MCF-7 or MCF-7/ADR cells. RESULTS APB reversed MCF-7/ADR multidrug resistance at the concentration of 10 μmol/L. Treated with 0, 5, 10, 20 μmol/L of ABP, P-gp gene and protein levels were suppressed significantly in a concentration-dependent manner. Furthermore, APB in 10 μmol/L markedly inhibited the function of P-gp. CONCLUSION APB potent reversed the multidrug resistance in MCF-7 ADR-resistant subline. The mechanism referred to P-gp inhibition.

关 键 词：Alopecurone B 阿霉素 乳腺癌 多药耐药 P-糖蛋白 

分 类 号：R285.5[医药卫生—中药学]