珠三角地区人群血浆中邻苯二甲酸酯的暴露水平研究  

Investigation on Exposure Levels of Phthalates in Human Plasma Collected from the Pearl River Delta

作　　者：陶斯湄 朱冬林[2] 陈艳华 吴惠勤[4] 周熙 徐长志[2] 谢芝芝 陶玲[1] 席云[2] TAO Si-mei;ZHU Dong-lin;CHEN Yan-hua;WU Hui-qin;ZHOU Xi;XU Chang-zhi;XIE Zhi-zhi;TAO Ling;XI Yun(Department of Pharmacy,The Third Affiliated Hospital of Sun Yat-sen University,Guangzhou 510630,China;Department of Clinical Laboratory,The Third Affiliated Hospital of Sun Yat-sen University,Guangzhou 510630,China;Guangzhou Borcon Biotechnology Co.,Ltd.,Guangzhou 510440,China;Guangdong Provincial Public Laboratory of Analysis and Testing Technology,Guangdong Provincial Key Laboratory of Emergency Test for Dangerous Chemicals,Guangdong Institute of Analysis,Guangzhou 510070,China)

机构地区：[1]中山大学附属第三医院药剂科,广东广州510630 [2]中山大学附属第三医院检验科,广东广州510630 [3]广州博康生物技术有限公司,广东广州510440 [4]广东省测试分析研究所广东省化学危害应急检测技术重点实验室,广东省分析测试技术公共实验室,广东广州510070

出　　处：《分析测试学报》2019年第11期1315-1320,共6页Journal of Instrumental Analysis

摘　　要：采用高效液相色谱-串联质谱(HPLC-MS/MS)法对珠三角地区人群血浆中16种邻苯二甲酸酯(PAEs)的暴露情况进行分析。样品预处理采用乙腈沉淀蛋白,正己烷液液萃取。结果表明,血浆中检出邻苯二甲酸二甲酯(DMP)、邻苯二甲酸二异丁酯(DiBP)、邻苯二甲酸二正丁酯(DnBP)、邻苯二甲酸丁基苄基酯(BBP)、邻苯二甲酸二环己基酯(DCHP)与邻苯二甲酸二(2-乙基己基)酯(DEHP)。其中DEHP的检出率为100%;其次为DiBP、DnBP与DMP,检出率分别为98.0%、62.0%和49.0%;DCHP与BBP的检出率最低,均为5.00%。∑PAEs的含量为12.4~1399 ng/g,中值与平均值分别为39.8、57.7 ng/g。6种PAEs中DEHP占比最高,为90.01%~99.96%。对不同性别与年龄人群的PAEs暴露水平进行研究,发现女性与低年龄组(≤40岁)人群血浆中的PAEs浓度较高,但仅DMP存在显著性差异。A high performance liquid chromatography-tandem mass spectrometric(HPLC-MS/MS)method was developed for the investigation on exposure levels of 16 phthalates(PAEs)in human plasma collected from the Pearl River Delta.Plasma samples were pretreated by protein precipitation with acetonitrile,and followed by liquid-liquid extraction with n-hexane.Results demonstrated that 6 PAEs,i.e.dimethyl phthalate(DMP),diisobutyl phthalate(DiBP),di-n-butyl phthalate(DnBP),butyl benzyl phthalate(BBP),dicyclohexyl phthalate(DCHP)and di-(2-ethyl hexyl)phthalate(DEHP)were detected in plasma samples.The detection rates for DEHP,DiBP,DnBP,DMP,DCHP and BBP were 100%,98.0%,62.0%,49.0%,5.00%and 5.00%,respectively.Contents for∑PAEs were in the range of 12.4-1399 ng/g,in which median and mean were 39.8 ng/g and 57.7 ng/g,respectively.DEHP occupied the highest ratio among the 6 PAEs,which was in the range of 90.01%-99.96%.By comparing the concentrations of PAEs in different sex and age groups,PAEs in female and less than 40 years old groups were detected with higher concentrations,and significant differences occured only in DMP concentrations of different sex and age groups.

关 键 词：邻苯二甲酸酯 珠三角地区 血浆 高效液相色谱-串联质谱 

分 类 号：O657.7[理学—分析化学] O657.63[理学—化学]