基于聚多巴胺纳米粒子的荧光增强型探针检测乙酰胆碱酶  

Detection of Acetylcholinesterase Using a Turn-on Fluorescence Probe Based on Polydopamine Nanoparticles

作　　者：杜方凯 李梦汝 莫远健 谭学才 黄乃阳 黄安娜 DU Fang-kai;LI Meng-ru;MO Yuan-jian;TAN Xue-cai;HUANG Nai-yang;HUANG An-na(Key Laboratory of Guangxi Colleges and Universities for Food Safety and Pharmaceutical Analytical Chemistry,Guangxi Key Laboratory of Chemistry and Engineering of Forest Products,School of Chemistry and Chemical Engineering,Guangxi University for Nationalities,Nanning 530006,China)

机构地区：[1]广西民族大学化学化工学院广西高校食品安全与药物分析化学重点实验室广西林产化学与工程重点实验室

出　　处：《分析测试学报》2019年第11期1347-1352,共6页Journal of Instrumental Analysis

基　　金：国家自然科学基金(11904059);广西省自然科学基金(2018GXNSFAA294044,2017GXNSFBA198026);广西省科技计划项目(AB18126048);广西高校中青年教师基础能力提升项目(2018KY0168)

摘　　要：以多巴胺盐酸盐为原料,在碱性条件下通过氧化反应制备聚多巴胺荧光纳米粒子(F-PDA),再与二氧化锰(MnO2)纳米片进行复合,构建了用于检测乙酰胆碱酶(AChE)的F-PDA@MnO2复合物荧光探针。MnO2纳米片和F-PDA复合,体系的荧光被猝灭。在底物乙酰硫代胆碱(ATCh)存在下,加入AChE后,体系荧光恢复,恢复程度与AChE浓度在5.0~100 mU/mL范围内呈良好的线性关系,检出限为0.14 mU/mL(S/N=3)。该方法用于缓冲溶液中AChE的检测,加标回收率为89.5%~120%,相对标准偏差为1.6%~2.5%,且具有较高的选择性。可为基于F-PDA传感体系构建提供新的方法学模型。Polydopamine fluorescent nanoparticles(F-PDA)were prepared with dopamine hydrochloride by oxidation under alkaline conditions.The F-PDA@MnO2 complex was prepared by combining F-PDA with manganese dioxide(MnO2)nanosheets.Based on this,a fluorescent probe based on F-PDA@MnO2 complex was constructed for the detection of acetylcholinesterase(AChE)as the fluorescence of F-PDA could be quenched by adding MnO2 nanosheets.In the presence of acetylthiocholine iodide(ATCh),the fluorescence of F-PDA was restored after adding AChE.Results showed that there was a good linear relationship for fluorescence recovery of the probe with AChE in the concentration range of 5-100 mU/mL,and the detection limit(S/N=3)was 0.14 mU/mL.The probe was applied in the detection of AChE in buffer solution with high selectivity.The recoveries were in the range of 89.5%-120%with the relative standard deviations(RSD)of 1.6%-2.5%.The research results provide a new methodological model for the construction of sensing system based on F-PDA.

关 键 词：聚多巴胺纳米粒子 二氧化锰纳米片 荧光 乙酰胆碱酶 

分 类 号：O657.3[理学—分析化学]