甘薯近缘种三浅裂野牵牛核基因组密码子分析  

Analysis of nuclear genome codon in sweetpotato relatives Ipomoea trifida

作　　者：秦桢[1] 侯夫云[1] 张安[2] 李爱贤[1] 董顺旭[1] 王庆美[1] Qin Zhen;Hou Fuyun;Zhang An;Li Aixian;Dong Shunxu;Wang Qingmei(Crop Research Institute,Shandong Academy of Agricultural Sciences/Scientific Observing&Experimental Station of Tuber&Root Crops in Huang-Huai-Hai Region,Ministry of Agriculture,Jinan 250100,Shandong,China;Jiangsu Xuzhou Sweetpotato Research Center,Xuzhou 221131,Jiangsu,China)

机构地区：[1]山东省农业科学院作物研究所/农业部黄淮海薯类科学观测实验站,山东济南250100 [2]江苏徐州甘薯研究中心,江苏徐州221131

出　　处：《江苏师范大学学报（自然科学版）》2019年第3期26-30,共5页Journal of Jiangsu Normal University：Natural Science Edition

基　　金：国家重点研发计划资助项目(2018YFD1000701-5,2018YFD1000705);现代农业产业技术体系建设专项资金(CARS-10-B07);山东省现代农业产业技术体系薯类创新团队建设项目(SDAIT-16-04);山东省农业科学院作物研究所学科团队建设项目(CXGC2018E01)

摘　　要：三浅裂野牵牛(Ipomoea trifida)是甘薯的亲缘关系较近野生种之一.分析了三浅裂野牵牛的32 264个具有完整编码框的核基因组基因,长度为165~15 273个核苷酸.根据基因在根、茎、叶中的表达量,选择1 382个低表达基因和1 644个高表达基因.利用CodonW软件分析发现,总基因的密码子有效数(ENC)为53.04,低表达基因的为52.80,高表达基因的为52.01.在总基因、低表达基因和高表达基因中分别发现了3个、9个和5个高频密码子.对低、高表达基因密码子的偏好性分析发现,高表达基因的进化速度低于低表达基因的.研究丰富了植物基因密码子理论,为转基因研究中密码子的优化提供了依据.Ipomoea trifida is a diploid wild-type species of sweetpotato. The 32 264 nuclear genome genes with complete coding frames of I.trifida were analyzed in this study, with the length from 165 to 15 273 nucleotides. According to the expression levels of genes in roots, stems and leaves, 1 382 low-expression genes and 1 644 high-expression genes were selected. By using CodonW software analysis, the effective numbers of codons(ENC) were found to be 53.04, 52.80 and 52.01 in total gene, the low expression gene and the high expression gene, respectively. Three, nine and five high frequency codons were also found in total genes, low expression genes and high expression genes, respectively. At the same time, the preference analysis of codons for low and high expressed genes was found that the evolution rate of highly expressed genes was lower than that of low expressed genes. This study can enrich the theory of plant gene codons and provide a basis for the optimization of codons in transgenic research.

关 键 词：三浅裂野牵牛 高频密码子 基因进化 

分 类 号：S531[农业科学—作物学]