基于ITS序列西南地区不同居群薤白亲缘关系及其地理分布格局研究  

Genetic Relationship and Geographical Distribution Pattern of Different Allium macrostemon Populations in Southwest China Based on ITS Sequences

作　　者：莫忠妹 成宇[1] 石甜 赵财[1,2] MO Zhongmei;CHENG Yu;SHI Tian;ZHAO Cai(College of Life Sciences,Guizhou University,Guiyang 550025,China;Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region(Ministry of Education),Guiyang 550025,China)

机构地区：[1]贵州大学生命科学学院,贵阳550025 [2]贵州大学山地植物资源保护与种质创新教育部重点实验室,贵阳550025

出　　处：《西北植物学报》2019年第9期1573-1580,共8页Acta Botanica Boreali-Occidentalia Sinica

基　　金：贵州省中药材现代产业技术体系建设(GZCYTX-02);贵州省生物学国内一流建设学科开放基金(GNYL[2017]009);贵州省烟草专卖局项目(201401);贵州省科技厅基金(20142064);大学生创新创业训练计划(黔科合J字[2015]2064号);贵大校创字([2015]024)

摘　　要：该研究以西南地区分布的薤白(Allium macrostemon Bunge)为材料,对21个不同居群的薤白进行核糖体DNA的ITS序列测定,并结合GenBank相关序列,对27个不同居群薤白开展了系统分析,以探讨西南地区不同居群薤白的亲缘关系和生物地理分布格局。结果表明:(1)西南地区不同产区薤白nrDNA ITS序列长度介于607~638 bp之间,G+C含量为48%~52%,平均值为50%。(2)基于贝叶斯法和最大简约法构建的系统树均表明薤白为一单系群。(3)系统发育树分析结果显示,27个居群的薤白总体划分为a组和b组,其中位于西南地区东面的四川剑阁县、重庆巫溪县、贵州德江县和云南丘北县的薤白共同聚为a组,位于西南地区西面的四川汶川县、四川石棉县、四川江油市以及云南虎跳峡、云南昆明市、云南三坝的薤白归为b组。研究表明,nrDNA ITS序列可以鉴定不同地区薤白的亲缘关系。To explore the relationship and biogeographic distribution pattern of A. macrostemon in different areas of Southwest China, we compared and analyzed 27 nrDNA ITS sequences of A. macrostemon, of which 21 were newly generated and 6 from GenBank. The results showed:(1) the sequence length of the A. macrostemon Bunge varied from 607-638 bp and the GC content of 48%-52%, with an average 50%.(2) A. macrostemon is monophyletic basing on Bayesian inference and Maximum-parsimony analysis.(3) Phylogenetic analysis indicated that the 27 populations are generally divided into group a and group b. Among them, Jian’ge County, Sichuan, Wixi County, Chongqing, Dejiang County, Guizhou and Qiubei County, Yunnan, located in the east of Southwest China, are clustered into group a;Wenchuan, Shimian, Jiangyou of Sichuan Province and Hutiaoxia, Kunming, Sanba of Yunnan Province located in the west of Southwest China belongs to group b. The results suggested that nrDNA ITS sequence could identify the relationship of A. macrostemon in different regions.

关 键 词：薤白 NRDNA ITS序列 亲缘关系 西南地区 

分 类 号：Q789[生物学—分子生物学]