葱兰愈伤组织诱导及植株再生  

Callus induction and plant regeneration of Zephyranthes candida

作　　者：熊琴 付素静 鲁万贵 XIONG Qin;FU Sujing;LU Wangui(College of Agroforestry Engineering and Planning,Tongren University,Tongren 554300,China;Yutang Dong and Miao Nationalities Township Junior Secondary School,Tongren 554300,China;Tongren Gardens Bureau,Tongren 554300,China)

机构地区：[1]铜仁学院农林工程与规划学院,贵州铜仁554300 [2]铜仁市万山区鱼塘侗族苗族乡初级中学,贵州铜仁554300 [3]铜仁市园林局,贵州铜仁554300

出　　处：《北方农业学报》2019年第5期82-85,共4页Journal of Northern Agriculture

摘　　要：为了给园林绿化提供更多的优质种苗,以葱兰种子作为外植体进行组织培养。结果表明:低浓度的2,4-D单独使用对葱兰种子愈伤组织的诱导效果不明显,MS+2,4-D 0.2 mg/L处理葱兰种子愈伤组织诱导率仅为3.3%;复合使用6-BA后葱兰种子愈伤组织和丛生芽的诱导率明显增加,MS+2,4-D 0.4 mg/L+6-BA 0.2 mg/L处理葱兰种子愈伤组织诱导率为90.0%,MS+6-BA 1.0 mg/L+NAA 1.0 mg/L处理葱兰丛生芽诱导率为66.7%。结论为诱导葱兰种子愈伤组织最佳培养基是MS+2,4-D 0.4 mg/L+6-BA 0.2 mg/L,诱导葱兰丛生芽的最佳培养基是MS+6-BA 1.0 mg/L+NAA 1.0 mg/L。The seeds of Zephyranthes candida as the explants were cultured in vitro for providing a higher quality seedling to landscaping.The results showed that the single addition of a low concentration of 2,4-D to the medium was ineffective to induce callus,the callus induction rate was only 3.3%when treated with MS+2,4-d 0.2mg/L,while 2,4-D together with 6-BA in the medium was promoting induction of callus and multiple shoots obviously.The callus induction rate was 90%on medium of MS containing 0.4 mg/L 2,4-D and 0.2 mg/L 6-BA,while the multiple shoot induction rate was 66.7%on medium of MS containing 1 mg/L 6-BA and 1 mg/L NAA.The callus induction optimal media was MS+2,4-D 0.4 mg/L+6-BA 0.2 mg/L,and the multiple shoot induction optimal media was MS+6-BA 1 mg/L+NAA 1 mg/L.

关 键 词：葱兰 组织培养 愈伤组织 丛生芽 

分 类 号：S682.31[农业科学—观赏园艺]