纳米氧化铈颗粒对人肺腺癌耐顺铂细胞辐射敏感性的影响  

Effect of cerium oxide nanoparticles on radiosensitivity of a drug-resistant human lung adenocarcinoma cell line

作　　者：王春燕[1] 佟鹏[1] 李辰[1] 邵帅[1] 曲功霖[1] 苟巧[1] 王成芳 齐雪松[1] WANG Chunyan;TONG Peng;LI Chen;SHAO Shuai;QU Gonglin;GOU Qiao;WANG Chengfang;QI Xuesong(Key Laboratory of Radiological Protection and Nuclear Emergency,National Institute for Radiological Protection,Chinese Center for Disease Control and Prevention,Beijing 100088,China)

机构地区：[1]中国疾病预防控制中心辐射防护与核安全医学所辐射防护与核安全应急中国疾病预防控制中心重点实验室

出　　处：《癌变．畸变．突变》2019年第6期464-468,共5页Carcinogenesis,Teratogenesis & Mutagenesis

摘　　要：目的:研究纳米氧化铈颗粒对人肺腺癌耐顺铂细胞(A549/DDP)辐射敏感性的影响。方法:采用细胞增殖抑制实验确定γ射 线半数抑制剂量和纳米氧化铈的使用浓度,细胞集落形成实验检测γ射线和纳米氧化铈处理后的细胞存活情况。将细胞分为阴性 对照,γ射线照射组,纳米氧化铈低、中、高浓度组(分别加入0.000 5、 0.05和5 μmol/L的纳米氧化铈处理后,再进行照射),采 用流式细胞术进行细胞凋亡率、细胞周期和胞内pH值检测。结果:通过细胞增殖抑制实验,确定γ射线诱导A549/DDP细胞半数 抑制剂量为25 Gy。细胞集落形成实验观察发现,与照射组相比,纳米氧化铈低浓度组细胞的SF2降低、a/b比值和增敏比升高。 流式细胞术检测发现,纳米氧化铈低浓度组细胞凋亡率增加(t=5.42, P<0.05);纳米氧化铈低、中、高浓度组的S期阻滞明显(t= 3.14、4.08、 6.81,P<0.05);纳米氧化铈低、高浓度组的胞内pH值升高(t=2.86、 4.93, P<0.05)。结论:低浓度纳米氧化铈对于体 外培养的A549/DDP细胞具有一定的辐射增敏作用。OBJECTIVE:To study the effect of cerium oxide nanoparticles (CeO2) on radiosensitivity of A549/DDP. METHODS: The half maximal inhibitory exposure dose and concentration of CeO2 were determined by inhibitory rate of proliferation of A549/DDP. The cell colony-forming experiment was performed to observe changes of cellular surviving fractions between the radiation group after treated with different concentration of CeO2 groups and radiation only group. A549/DDP cells were grouped as negative control (control), radiation control (rad), and the low, medium and high concentration of CeO2 groups (final concentrations were 0.000 5, 0.05 and 5 μmol/L). Apoptosis,cell cycle and intracellular pH were determined using by flow cytometry. RESULTS: The half maximal inhibitory exposure dose of A549/DDP induced by γ-rays was 25 Gy,which was determined by inhibitory rate of A549/DDP proliferation. At the survival fraction of 2 Gy (SF2), the ratios of a/b and the sensitive enhancement ratios (SER) were obtained by the cell colony-forming experiment. Comparing with the rad group,the value SF2 of low concentration CeO2 group was decreased. The ratio of a/b and the value of SER of the low concentration CeO2 group were both increased. From the flow cytometry analyses,the percent of apoptosis in the low concentration CeO2 group was increased (t=5.42, P<0.05);S-phase arrest of the low,medium and high concentration CeO2 groups were obvious (t= 3.14, 4.08,6.81, P<0.05). The value of pH in cells of the low and high concentration CeO2 groups were raised (t=2.86, 4.93, P<0.05). CONCLUSION: All the results indicate that low concentrations of CeO2 enhanced radiosensitivity of A549/DDP.

关 键 词：纳米氧化铈颗粒 A549/DDP细胞 辐射敏感性 Γ射线 

分 类 号：R146[医药卫生—公共卫生与预防医学]