玉米弯孢叶斑病菌多聚半乳糖醛酸酶Clpg1基因功能验证  

Functional verification of polygalacturonase Clpg 1 gene in Curvularia lunata causing maize Curvalaria leaf spot

作　　者：崔佳[1] 赵丰舟 刘震 曲建楠 刘铜 左豫虎[1] CUI Jia;ZHAO Fengzhou;LIU Zhen;QU Jiannan;LIU Tong;ZUO Yuhu(College of Agronomy,Heilongjiang Bayi Agricultural University,Daqing 163319,China;Institute of Tropical Agriculture and Forestry,Hainan University,Haikou 570228,China)

机构地区：[1]黑龙江八一农垦大学农学院,大庆163319 [2]海南大学热带农林科学院,海口570228

出　　处：《植物保护》2019年第6期163-169,184,共8页Plant Protection

基　　金：海南大学高层次人才科研启动基金(KYQD(ZK)1851)

摘　　要：为明确玉米弯孢叶斑病菌的多聚半乳糖醛酸酶基因Clpg1在其致病过程中的作用,采用Double-joint PCR技术构建融合片段,利用PEG介导的原生质体转化技术获得18株抗潮霉素突变体,经检测得到1株Clpg1基因敲除突变体ΔClpg1。通过与野生型菌株比较,发现ΔClpg1产孢量和孢子萌发率下降、多聚半乳糖醛酸酶活性降低,致病力减弱。这些结果表明Clpg1基因影响多聚半乳糖醛酸酶活性,调控了玉米弯孢叶斑病菌的致病性。In order to clarify the role of polygalacturonase Clpg1 gene in the pathogenicity of Curvularia lunata, the fusion fragment was constructed using double-joint PCR, and 18 hygromycin-resistant mutants were obtained by using PEG-mediated protoplast transformation technique. A knock-out mutant ΔClpg1 which contained no Clpg1 gene was found. Compared with the wild-type strain, the sporulation yield and germination rate of ΔClpg1 decreased;the polygalacturonase activity was reduced, and the pathogenicity was weakened. These results indicate that the Clpg1 gene affects the polygalacturonase activity and regulates the pathogenicity of Curvularia lunata.

关 键 词：玉米弯孢叶斑病菌 Clpg1 基因敲除 生物学特性 致病性 

分 类 号：S432.1[农业科学—植物病理学]