氯化苦熏蒸对土壤反硝化作用及nirS型反硝化细菌群落结构的影响  

Effects of chloropicrin fumigation on soil denitrification and the community structure of nirS-denitrifying bacteria

作　　者：燕平梅[1,2] 魏爱丽 赵文婧[1,2] 李娜 李博[1,2] 曹坳程 YAN Pingmei;WEI Aili;ZHAO Wenjing;LI Na;LI Bo;CAO Aocheng(Department of Biology,Taiyuan Normal University,Taiyuan 030619,China;Strategic Alliance of Green Industry Technology Innovation for Soil Disinfestation and Activation,Taiyuan 030619,China;Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100193,China)

机构地区：[1]太原师范学院生物系,太原030619 [2]土壤消毒活化绿色产业技术创新战略联盟,太原030619 [3]中国农业科学院植物保护研究所,北京100193

出　　处：《植物保护》2019年第6期208-213,228,共7页Plant Protection

基　　金：现代农业产业技术体系北京市创新团队;国家自然科学基金(40871131;31240009)

摘　　要：探究氯化苦熏蒸对土壤反硝化作用的影响及机制。采用理化分析和DGGE方法分析了氯化苦熏蒸后土壤反硝化及nirS型反硝化细菌群落结构。不同浓度氯化苦熏蒸对土壤nirS型反硝化细菌群落均匀度无影响,而对nirS型反硝化细菌多样性和丰富度指数的影响为高浓度>中浓度>低浓度>对照。对土壤反硝化作用的影响表现为,经500 mg/kg氯化苦熏蒸的土壤在培养初期(0 d)反硝化率比对照降低10.55%,土壤反硝化作用被显著抑制。2周后3种浓度氯化苦均抑制土壤反硝化作用, 4周后土壤的反硝化作用缓慢恢复。500 mg/kg浓度氯化苦对土壤反硝化强度的影响与nirS型反硝化细菌群落结构变化不同,说明土壤反硝化作用的强弱并非完全决定于土壤nirS型反硝化细菌。The effects of chloropicrin fumigation on edaphic denitrification and the mechanisms were studied by using DGGE and physical and chemical analysis of soil denitrification and the community structure of nirS-denitrifying bacteria. Different concentrations of chloropicrin fumigation had no effect on the evenness of soil nirS-denitrifying bacterial community, but their effects on the diversity and abundance indexes of nirS-denitrifying bacteria were as followed: high concentration > medium concentration> low concentration > the control. The soil denitrification experiments showed that after treated by 500 mg/kg chloropicrin, at the initial stage of culture(0 d) the denitrification rate decreased by 10.55% compared with the control, denitrification were significantly inhibited in soil. After two weeks, all of the three concentrations of chloropicrin inhibited denitrification in soil, but the denitrification in soil naturally recovered after four weeks. The effect of 500 mg/kg chloropicrin on soil denitrification intensity was different from the change in the community structure of nirS-denitrifying bacteria, explaining why the strength of denitrification in soil was not completely determined by nirS-denitrifying bacteria.

关 键 词：氯化苦 土壤熏蒸剂 nirS 反硝化作用 DGGE 

分 类 号：S482.6[农业科学—农药学]