胃肠转流术改善糖尿病大鼠胰岛素抵抗和增加外周脂肪和肌肉组织中GLUT-4基因表达  

Gastric bypass surgery improves insulin resistance and increases GLUT-4 gene expression of peripheral adipose and skeletal muscle tissue in diabetic rats

作　　者：左莹[1] 刘菊华[1,2] 严宗逊 雷震[1] ZUO Ying;LIU Juhua;YAN Zongxun;LEI Zhen(Department of Internal Medicine,Affiliated Hospital of North Sichuan Medical Collage,Nanchong,Sichuan 637000,China;Chengdu University of Traditional Chinese Medicine,Chengdu,Sichuan 610000,China)

机构地区：[1]川北医学院附属医院内科,四川南充637000 [2]成都中医药大学,成都610000

出　　处：《重庆医学》2019年第22期3790-3793,共4页Chongqing medicine

基　　金：四川省卫生厅科研项目(120455);川北医学院科研项目(CBY-11-A-QN17);南充市市校合作科研项目(18SXHZ0301)

摘　　要：目的观察并分析胃肠转流术对2型糖尿病(T2DM)大鼠血糖、胰岛素抵抗指数及葡萄糖转运蛋白4(GLUT-4)基因表达的影响及可能机制。方法通过高脂饮食及腹腔注射链脲佐菌素制备T2DM大鼠模型,成模后分为T2DM胃肠转流术组(DRO组)和T2DM假手术组(DSO组),SD大鼠胃肠转流术组(NRO组)和SD大鼠假手术组(NSO组)。测定术前、术后4周、术后8周各组空腹血糖(FBG)、空腹血清胰岛素(Fins)及胰岛素抵抗指数(IR);术后8周处死大鼠,取外周脂肪和肌肉组织检测GLUT-4基因表达。结果DRO组FBG及HOMA-IR术后4周较术前下降,但差异无统计学意义(P>0.05),术后8周较术前明显下降(P<0.05),同时显著低于同时间点DSO组(P<0.05),DRO组FBG仍高于NRO及NSO组(P<0.05),但HOMA-IR与NRO及NSO组相比差异无统计学意义(P>0.05)。术后8周,DRO组外周脂肪及肌肉组织中GLUT-4基因表达较DSO组明显增加(P<0.01),与NRO及NSO组相比差异无统计学意义(P>0.05)。Pearson相关性分析提示术后8周外周脂肪及肌肉组织中GLUT-4基因表达与FBG及HOMA-IR呈负相关(P<0.01)。结论胃肠转流术可改善T2DM大鼠的胰岛素抵抗、降低血糖,其机制可能与术后脂肪组织和肌肉组织中GLUT-4基因表达增高相关。Objective To observe and analyze the influence of gastric bypass surgery on blood glucose,insulin resistance index and glucose transporter 4(GLUT-4)gene expression in type 2 diabetes mellitus(T2DM)rats and its possible mechanism.Methods The T2DM rat model was prepared by high fat diet and intraperitoneal injection of streptozotocin.After modeling,the T2DM rats were divided into the T2DM gastric bypass surgery group(DRO group)and T2DM sham operation group(DSO group),while the SD rats were also divided into the gastric bypass surgery group(NRO group)and SD rats sham operation group(NSO group).Fasting plasma glucose(FBG),fast plasma insulin(Fins)and the insulin resistance index(HOMA-IR)were measured before operation and at postoperative 4,8 weeks.The rats were killed at postoperative 8 weeks,the peripheral adipose and skeletal muscle tissues were taken for detecting the expression of GLUT-4 gene.Results FBG and HOMA-IR at postoperative 4 weeks in the DRO group was decreased compared with those before operation,but the difference had no statistical significant(P>0.05),which at postoperative 8 weeks were significantly decreased compared with those before operation,meanwhile was significantly lower than those at the same time point in the DSO group(P<0.01),FBG in the DRO group was still higher than that in the NRO group and NSO group(P<0.05),but HOMA-IR had no statistical difference between the NRO group and NSO group(P>0.05).At postoperative 8 weeks,the expression GLUT-4 gene in adipose and skeletal muscle tissues of the DRO group was significant increased compared with DSO group(P<0.05),but which had no statistical difference between the NRO group and NSO group(P>0.05).The Pearson correlation analysis demonstrated that the expression of GLUT-4 gene in adipose and skeletal muscle tissues was negatively correlated with FBG and HOMA-IR(P<0.01).Conclusion The gastric bypass surgery can improve the insulin resistance and decrease blood glucose in T2DM rats,its mechanism may be correlated with the increase of GLUT-4 gene e

关 键 词：2型糖尿病 胃肠转流术 血糖 胰岛素抵抗 葡萄糖转运蛋白4基因 

分 类 号：R587.1[医药卫生—内分泌]