50个国外小麦品种(系)抗叶锈性鉴定  

Plant Resistance to Leaf Rust in 50 Foreign Wheat Cultivars

作　　者：焦悦 王思曼 赵喜兰 张培培[1] 郑慧敏 李在峰[1] 刘大群[1,2] JIAO Yue;WANG Siman;ZHAO Xilan;ZHANG Peipei;ZHENG Huimin;LI Zaifeng;LIU Daqun(College of Plant Protection,Agricultural University of Hebei/Biological Control Center of Plant Disease and Plant Pests of Hebei Province,Baoding 071001,China;Graduate School of Chinese Academy of Agricultural Science,Beijing 100081,China)

机构地区：[1]河北农业大学植物保护学院/河北省农作物病虫害生物防治工程技术研究中心,河北保定071001 [2]中国农业科学院研究生院,中国北京100081

出　　处：《河南农业科学》2019年第12期79-88,共10页Journal of Henan Agricultural Sciences

基　　金：国家自然科学基金项目(31571662,31601299);农业部“引进国际先进农业科学技术”项目(2016-X16)

摘　　要：为确定小麦品种(系)所携带的抗病基因,对来自国外的50个小麦品种(系)进行苗期抗叶锈病基因推导和成株期抗叶锈鉴定。在苗期,根据供试品种(系)与20个毒力不同的叶锈菌生理小种的互作反应,与36个已知抗叶锈基因载体品种的侵染型进行比较,同时对试验材料进行连续2a两点的田间抗锈性试验,并利用分子标记进一步检测供试品种(系)中含有的抗叶锈病基因。基因推导和标记检测结果表明,Insijnia、Palpich、MV laura、Mason/jagger、Re7145共5个小麦品种(系)含有Lr 1,Insijnia、Tx03a0148、F98047j14-2inc、T67/X84w063-9-45//K92、Mason/jagger共5个小麦品种(系)含有Lr 26,Hk1/6/Nvsr3/5bez/tvr、Tx03a0148、Palpich、Kanto107、MV laura、F92080g1-1/F93042g2-1、Mv05-08、Norin61、Bruta、Aca801、F98047j14-2inc、T67/X84w063-9-45//K92、Mason/jagger共13个小麦品种(系)含有成株慢锈基因Lr 34,Nidera baguette 10、Insijnia、Nsa09-3645、Soissons、Aztec、Carimulti、Mason/jagger、Re714、Kniish-46、Nuwest/4/D887-74/pew/、Fr03733共11个小麦品种(系)含有成株抗性基因Lr 37,Sagittario、Hk1/6/Nvsr3/5bez/tvr、Insijnia、Fr03717、Dorico等共45个品种(系)含有成株慢锈基因Lr 46,T67/X84w063-9-45//K92、Re7145共2个品种(系)可能含有Lr 18,Fr03724、Fr3713、T67/X84w063-9-45//K92共3个品种(系)可能含有Lr 21,T67/X84w063-9-45//K92仅1个品种(系)可能含有Lr 36。田间试验结果表明,Mv05-08、Fr03725、Re714、Fr03717、Fr03724等共19个品种(系)表现慢锈性。A total of 50 introduced wheat cultivars were used for identifying seedling resistance gene for leaf rust at seedling stage and slow rusting resistance at adult stage.At seedling stage 20 Puccinia triticina pathotypes with virulent genes were used to inoculate the 50 introduced cultivars and 36 differential cultivars with known leaf rust resistance genes for postulating seedling genes present in these cultivars.Molecular marker were also used to test the cultivars for further confirming the results based on gene postulation.These cultivars were also tested for slow rusting resistance in field in two consecutive years at two locations.The results based on gene postulation and molecular marker detection showed that each of Lr 1 and Lr 26 were identified in five cultivars.Insijnia,Palpich,MV laura,Mason/jagger and Re7145 contained Lr 1,while Lr 26 were found in Insijnia,Tx03a0148,F98047j14-2inc,T67/X84w063-9-45//K92 and Mason/jagger.Based on gene postulation and molecular marker,Lr 34 were detected in 13 cultivars(Hk1/6/Nvsr3/5bez/tvr,Tx03a0148,Palpich,Kanto107,MV laura,F92080g1-1/F93042g2-1,Mv05-08,Norin61,Bruta,Aca801,F98047j14-2inc,T67/X84w063-9-45//K92 and Mason/jagger)and Lr 37 were present in 11 cultivars(Nidera baguette 10,Insijnia,Nsa09-3645,Soissons,Aztec,Carimulti,Mason/jagger,Re714,Kniish-46,Nuwest/4/D887-74/pew/and Fr03733).Lr 46 was detected in 45 cultivars(Sagittario,Hk1/6/Nvsr3/5bez/tvr,Insijnia,Fr03717,Dorico etc.).Lr 18 was present in two cultivars(T67/X84w063-9-45//K92 and Re7145)and three cultivars(Fr03724,Fr3713 and T67/X84w063-9-45//K92)contained Lr 21.Lr36 was found only in T67/X84w063-9-45//K92.19 cultivars(Mv05-08,Fr03725,Re714,Fr03717,Fr03724 etc.)slowed rusting resistance in the field trials.

关 键 词：国外小麦种质 小麦叶锈病 基因推导 苗期鉴定 成株期鉴定 抗叶锈基因 

分 类 号：S435[农业科学—农业昆虫与害虫防治]