罗格列酮对哮喘小鼠气道炎症因子及MUC5ac蛋白表达水平的影响  

Effects of Rosiglitazone on Airway Inflammatory Factors and MUC5ac Protein Expression in Asthmatic Mice

作　　者：杨红霞[1] 张建勇[1] 赵建军[1] 陈玲[1] 郑飞彦 毕海 张红[1] YANG Hongxia;ZHANG Jianyong;ZHAO Jianjun;CHEN Ling;ZHENG Feiyan;BI Hai;ZHANG Hong(The Second Department of Respiratory Medicine,Affiliated Hospital of Zunyi Medical College,Zunyi 563003,China)

机构地区：[1]遵义医学院附属医院呼吸二科

出　　处：《医药导报》2019年第12期1562-1566,共5页Herald of Medicine

基　　金：贵州省科学技术基金项目(黔科合J字[2013]2329号);贵州省科技计划课题(黔科合SY字[2015]3050)

摘　　要：目的探讨罗格列酮灌胃对哮喘小鼠气道炎症因子及MUC5ac蛋白表达水平影响。方法雄性BALB/c小鼠30只,随机分为正常对照组、哮喘组、罗格列酮组,每组各10只。各组肺组织采用伊红(HE)染色观察气道炎症,阿尔新蓝-碘酸雪夫(AB-PAS)染色观察气道上皮杯状细胞和黏液物质改变,采用酶联免疫吸附(ELISA)法检测支气管肺泡灌洗液(BALF)中活性因子[白细胞介素(IL)-5、IL-13、肿瘤坏死因子(TNF)-α],免疫组化(IHC)检测肺组织MUC5ac蛋白表达水平,实时荧光定量(RT-PCR)检测肺组织MUC5ac mRNA表达水平。结果罗格列酮组小鼠支气管周围炎症病理评分、气道上皮杯状细胞和黏液物质阳性相对着色面积均较哮喘组降低,但仍高于正常对照组(P<0.01)。罗格列酮组小鼠BALF中IL-5、IL-13及TNF-α水平较哮喘组降低,但仍高于正常对照组(P<0.01)。罗格列酮组小鼠肺组织MUC5ac蛋白和mRNA含量较哮喘组降低,但仍高于正常对照组(P<0.05)。结论罗格列酮可有效减轻哮喘小鼠气道炎症反应,调节气道黏蛋白MUC5ac的表达,抑制气道黏液高分泌。Objective To study the effects of rosiglitazone on airway inflammatory factors and MUC5 ac expression in asthmatic mice. Methods Thirty male BALB/c mice were randomly divided into normal control group, asthma group and rosiglitazone group(10 rats in each group). Airway inflammation was observed by HE staining in lung tissue of each group. The changes of goblet cells and mucus in airway epithelium were observed by Alxin blue-periodic acid Schiff(AB-PAS) staining. The inflammatoryfactors(IL-5,IL-13,TNF-α) in bronchoalveolar lavage fluid(BALF) were detected by ELISA method. The expression of MUC5 ac in lung tissue was detected by immunohistochemistry(IHC) method. The expression of MUC5 ac mRNA in lung tissue was detected by real-time fluorescence quantitative PCR(RT-qPCR). Results The pathological score of peribronchial inflammation, the positive relative staining area of goblet cells and mucus in airway epithelium were lower than those in asthma group, but still higher than those in normal control group(P<0.01). The levels of IL-5, IL-13 and TNF-α in BALF in rosiglitazone group were lower than those in asthma group, but still higher than those in normal control group(P<0.01). The content of MUC5 ac protein and mRNA in lung tissue of rosiglitazone group was lower than that of asthma group, but still higher than that of normal control group(P<0.05). Conclusion Rosiglitazone can effectively attenuate the airway inflammatory reaction of asthmatic mice, regulate the expression of airway mucin MUC5 ac and inhibit airway mucus hypersecretion.

关 键 词：罗格列酮 哮喘 炎症因子 MUC5ac蛋白 

分 类 号：R974.3[医药卫生—药品] R965[医药卫生—药学]