蛇足石杉不同天然居群的土壤酶活性研究  

Studies on Enzymes of Soil from Different Natural Populations of Huperzia serrata

作　　者：王德立[1] 齐耀东[1] WANG De-li;QI Yao-dong(Hainan-branch Institute of Medicinal Plant Development,Peking Union Medical College&Chinese Academy of Medical Sciences,Haikou 570311,China;Institute of Medicinal Plant Development,Peking Union Medical College&Chinese Academy of Medical Sciences,Beijing 100193,China)

机构地区：[1]中国医学科学院北京协和医学院药用植物研究所海南分所

出　　处：《中国现代中药》2019年第10期1407-1410,1414,共5页Modern Chinese Medicine

基　　金：中央本级重大增减支项目(2060302);国家自然科学基金项目(81303159)

摘　　要：目的:了解可能促进蛇足石杉孢子萌发及植株生长的土壤酶种类及其活性情况。方法:分别采集重庆黄水镇蛇足石杉分布区中5年、10年、15年生柳杉林地土壤,海南霸王岭东五样地和东二样地土壤。采用酶联免疫法测定土壤中蛋白酶、过氧化氢酶、多酚氧化酶、脲酶、蔗糖酶、纤维素酶和磷酸酶7种酶活性。结果:重庆15年林地、海南东五等有蛇足石杉分布的样地中重庆15年林地样地的蛋白酶、过氧化氢酶及多酚氧化酶活性较高,而海南样地脲酶、蔗糖酶活性较高;重庆15年林地土壤蛋白酶、过氧化氢酶及磷酸酶活性高于5年和10年林地,而多酚氧化酶、脲酶、蔗糖酶活性低于二者;5年林地土壤纤维素酶活性较高;海南东五样地土壤蛋白酶、蔗糖酶、纤维素酶及磷酸酶活性低于东二样地,两样地在过氧化氢酶、多酚氧化酶及脲酶活性方面差异不显著。结论:蛋白酶、过氧化氢酶和多酚氧化酶可能有利于蛇足石杉孢子萌发;较高的脲酶、蔗糖酶、纤维素酶和磷酸酶活性可能更有利于蛇足石杉植株生长。Objective:To find the soil enzymes which promote spores germination and growth of Huperzia serrata.Methods:The soil was collected from the following 6 plots:5 years old,10 years old and 15 years old Cryptomeria woods in Huangshui town,Chongqing province;the plots of Donger and Dongwu in Ba Wangling Natural reserve,Hainan province.The level of seven soil enzymes activity was detected by ELISA,and those were protease,catalase,polyphenol oxidase enzyme(PPO),urease,invertase,cellulase and phosphatase.Results:In natural populations of H.serrata,the enzyme activity of soil protease,catalase and PPO from 15 years old woods plot were higher,but the activity of urease and invertase was lower than Hainan Dongwu plot.In three Chongqing plots,the activity of protease,catalase and phosphatase of 15 years old woods plot was higher than that of 5 years and 10 years woods plots,but the activity of PPO,urease and invertase was lower.The cellulase activity of 15 years old woods was higher than that of other two plots.In Hainan plots,the activity of protease,invertase,cellulose and phosphatase of Dongwu was higher than Donger.The activity difference of catalase,PPO and urease was not significant between Dongwu and Donger plots.Conclusion:Protease,catalase and PPO maybe accelerated spores of H.serrata germination.The plants will grow well in the surrounding with higher activity of urease,invertase,cellulase and phosphatase.

关 键 词：蛇足石杉 土壤酶 孢子萌发 天然居群 样地 

分 类 号：R282.71[医药卫生—中药学]