HPLC测定不同地区桑椹饮片中七种成分含量  

Simultaneous Determination of Seven Components in Mori Fructus Slice from Different Areas of China by HPLC

作　　者：常艳旭[1] 王涛 李晋[1] 何俊[1] 金华[2] 马琳[2] 杨雪晶 CHANG Yan-xu;WANG Tao;LI Jin;HE Jun;JIN Hua;MA Lin;YANG Xue-jing(Tianjin State Key Laboratory of Mordern Chinese Medicine,Tianjin University of TCM,Tianjin 300193,China;College of Chinese Materia Medica,Tianjin University of TCM,Tianjin 300193,China;School of Pharmacy,Harbin University of Commerce,Harbin 150076,China)

机构地区：[1]天津中医药大学/天津市现代中药重点实验室,天津300193 [2]天津中医药大学中药学院,天津300193 [3]哈尔滨商业大学药学院,黑龙江哈尔滨150076

出　　处：《中国现代中药》2019年第10期1411-1414,共4页Modern Chinese Medicine

基　　金：中央本级重大增减支项目(2060302);大学生创新创业训练计划项目(201510063026)

摘　　要：目的:建立同时测定桑椹饮片中新绿原酸、绿原酸、隐绿原酸、芦丁、异槲皮苷、紫云英苷和槲皮素7种成分含量的高效液相色谱(HPLC)测定方法,为评价中国不同地区桑椹饮片的质量提供技术手段。方法:采用ODS-C18(250 mm×4.6 mm,5μm)色谱柱,水(含0.2%甲酸)-乙腈为流动相,梯度洗脱,流速为1 mL·min-1,检测波长为360 nm。结果:新绿原酸、绿原酸、隐绿原酸、芦丁、异槲皮苷、紫云英苷和槲皮素7种成分线性关系良好,加样回收率在95.0%~105%。不同地区使用的桑椹饮片中7种成分含量差异较大。结论:本研究建立了一种稳定、可靠的桑椹饮片中7种成分含量同时测定的高效液相色谱方法,为桑椹饮片质量研究提供参考。Objective:To establish an HPLC-UV method for simultaneous determination of neochlorogenic acid,chlorogenic acid,cryptochlorogenic acid,rutin,quercetin,astragaline and quercetin for quality evaluation of Mori Fructus slice.Methods:The analysis was carried out on an ODS-2 column(250 mm×4.6 mm,5μm)column with water(containing 0.2%formic acid)-acetonitrile as the mobile phase by RP-HPLC method.The detection wavelength was 360 nm and flow rate was 1 mL·min-1.Results:Seven components showed respectively a good linear relationship.The recoveries were 95.0%~105%.The contents of seven components in Mori Fructus slice from different areas was quite different.Conclusion:The simple and accurate HPLC-UV method was established and validated to determine the contents of seven components for quality evaluation of Mori Fructus.

关 键 词：桑椹 高效液相色谱法 含量测定 

分 类 号：R286[医药卫生—中药学]