春兰‘黄梅’ב黄荷’杂交F1根状茎的组培快繁研究  

Tissue Culture for Rapid Propagation of A Cymbidium goeringii Hybrid

作　　者：漆子钰 周育真[1] 艾叶 彭东辉[1] QI Zi-yu;ZHOU Yu-zheng;AI Ye;PENG Dong-hui(College of Landscape Architecture,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China;Yichun Garden Bureau,Yichun,Jiangxi 336000,China)

机构地区：[1]福建农林大学园林学院,福建 福州 350002 [2]江西省宜春市园林局,江西 宜春 336000

出　　处：《福建农业学报》2019年第9期1032-1039,共8页Fujian Journal of Agricultural Sciences

基　　金：福建省种业创新与产业化工程林业项目(ZYCX-LY-2017005)

摘　　要：【目的】筛选春兰组培各阶段的最佳培养条件,建立优良的春兰快繁体系,为春兰大规模生产开发提供科学依据。【方法】以春兰‘黄梅’ב黄荷’F1无菌播种形成的根状茎为试验材料,通过基本培养基(1/2MS、改良1/2MS、HyponexⅡ)和植物生长调节剂(6-BA、NAA、TDZ、IBA)等因子不同组合,围绕增殖、分化、生根等阶段建立其再生体系,并对培育出的组培苗进行移栽练苗。【结果】增殖最适培养基为3.0 g·L^-1 HyponexⅡ+0.5 mg·L^-16-BA+3.0 mg·L^-1 NAA+1.0 g·L^-1活性炭(AC)+30.0 g·L^-1白砂糖(Su)+7.0 g·L^-1琼脂(Ag),生长速度和增殖系数分别为7.31和6.02;分化最适培养基为2.0 mg·L^-16-BA+0.3 mg·L^-1 NAA+30.0 g·L^-1 Su+7.0 g·L^-1 Ag,分化率、芽诱导个数和株高分别为90.00%、5.44个和2.53 cm;生根最适培养基为1.5 mg·L^-1 IBA+2.0 g·L^-1蛋白胨+1.0 g·L^-1 AC+25.0 g·L^-1 Su+7.0 g·L^-1 Ag,生根率、生根数和平均根长分别为100.00%、8.03条和3.00 cm;春兰组培苗练苗移栽60 d后存活率达96.53%。【结论】新型基本培养基HyponexⅡ(3.0 g·L^-1)对春兰增殖培养有高效促进作用,高水平的IBA(1.5 mg·L^-1)有利于春兰组培苗生根壮苗。【Objective】 Optimum conditions for an efficient, rapid propagation from rhizomes of a Cymbidium goeringii hybrid were investigated.【Method】 Rhizomes of the aseptically seeded F1 hybrid between two C. goeringii cultivars, Huangmei and Huang-he, were cultivated on various media(i.e., 1/2 MS、modified 1/2 MS, and HyponexⅡ) with added plant growth regulators(i.e., 6-BA, NAA, TDZ, and/or IBA) to monitor the plant growth throughout propagation, differentiation, and rooting of the regeneration process. The seedlings were subsequently transplanted outdoor to determine their survival rate.【Result】 In the propagation stage, the optimal medium was 3.0 g HyponexⅡ·L^-1 + 0.5 mg 6-BA·L^-1 + 3.0 mg NAA·L^-1 + 1.0 g activated carbon(AC)·L^-1 + 30.0 g sugar(Su)·L^-1 + 7.0 g agar(Ag)·L^-1 to achieve a growing rate of 7.31 and a multiplication coefficient of 6.02. The optimal medium for differentiation was 2.0 mg 6-BA·L^-1 + 0.3 mg NAA·L^-1 + 30.0 g Su·L^-1 + 7.0 g Ag·L^-1 to deliver an averaged 90.00% differentiation, 5.44 buds per rhizome segment, and 2.53 cm height of seedling. For optimum rooting, the medium was 1.5 mg IBA·L^-1 + 2.0 g peptone·L^-1 + 1.0 g AC·L^-1 + 25.0 g Su·L^-1 + 7.0 g Ag·L^-1 to result in100.00% root-generation with an averaged 8.03 roots at 3.00 cm long per plant. The survival rate of plantlets 60 d after transplantation was up to 96.53%.【Conclusion】 HyponexⅡ applied at 3.0 g·L^-1 was found highly efficient for the C.goeringii rhizome regeneration;and, the addition of 1.5 mg·L^-1 of IBA in the medium significantly enhanced the rooting for the seedlings.

关 键 词：春兰 增殖培养 分化培养 生根培养 

分 类 号：S68[农业科学—观赏园艺]