乙酰肝素酶和整合素α5β1蛋白在葡萄胎组织中的表达及其临床意义  

Expressions of heparanase and integrin α5β1 proteins in hydatidform mole tissue and their clinnical significances

作　　者：王闯[1] 于淑莉 李桃[3] 呂蓉蓉 齐洁敏[1] WANG Chuang;YU Shuli;LI Tao;LYU Rongrong;QI Jiemin(Department ofPathology,Chengde Medical College,Chengde 067000,China;Department ofPathology,Chengde Women and Children Health Hospital,Chengde 067000,China;Department ofPathology,First Hospital,Qinhuangdao City,Qinhuangdao 066000,China)

机构地区：[1]承德医学院病理教研室,河北承德067000 [2]河北省承德市妇幼保健院病理科,河北承德067000 [3]河北省秦皇岛市第一医院病理科,河北秦皇岛066000

出　　处：《吉林大学学报（医学版）》2019年第6期1361-1366,I0020,共7页Journal of Jilin University：Medicine Edition

基　　金：河北省科技厅科技支撑项目资助课题(15277703D);河北省高校重点学科建设项目资助课题[冀教高(2013)4号]

摘　　要：目的:观察乙酰肝素酶(HPA)蛋白和整合素α5β1蛋白在人良、恶性葡萄胎组织中的表达情况,阐明其在良、恶性葡萄胎发生发展中的作用。方法:选择人正常胎盘绒毛组织50例(正常胎盘绒毛组)、良性葡萄胎组织40例(良性葡萄胎组)和恶性葡萄胎组织17例(恶性葡萄胎组)。采用免疫组织化学SP法检测各组组织中HPA和整合素α5β1蛋白的阳性表达率和阳性表达强度,Western blotting法分别检测各组组织中HPA和整合素α5β1蛋白表达水平,Spearman等级相关分析法分析良、恶性葡萄胎组织中HPA蛋白和整合素α5β1蛋白表达的相关性。结果:良性葡萄胎组和恶性葡萄胎组组织中HPA和整合素α5β1蛋白阳性表达率、阳性表达强度和表达水平高于正常胎盘绒毛组(P<0.05);恶性葡萄胎组组织中HPA和整合素α5β1蛋白阳性表达率、阳性表达强度和表达水平高于良性葡萄胎组(P<0.05)。在良、恶性葡萄胎组织中HPA蛋白与整合素α5β1蛋白的表达均呈正相关关系(r=0.506,P<0.05;r=0.555,P<0.05)。结论:联合检测HPA和整合素α5β1蛋白的表达可成为良性葡萄胎和恶性葡萄胎鉴别诊断的预测指标。Objective:To observe the expressions of heparanase(HPA)Protein and integrinα5β1Protein in the human benign and malignant hydatidform mole tissues,and to elucidate their roles in the occurrence and development of benign and malignant hydatidiform moles.Methods:A total of 50 cases of normalPlacental villus tissue(normalPlacental villus group),40 cases of benign hydatidiform moles tissue(benign hydatidform mole group)and 17 cases of malignant hydatidiform mole tissue(malignant hydatidform mole group)were selected.ThePositive expression rates and expression intensities of HPAProtein and integrinα5β1Protein in tissues in various groups were detected by immunohistochemical SP method,the expression levels of HPAProtein and integrinα5β1Proteins in tissues in various groups were detected by Western blotting method;Spearman correlation method was used to analyze the correlation between the expressions of HPAProtein and integrinα5β1Protein.Results:ThePositive expression rates,the expression intensities and the expression levels of HPA and integrinα5β1 in tissues in benign hydatidiform mole group and malignant hydatidiform mole group were higher than those in normalPlacental villus group(P<0.05);thePositive expression rate,the expression intensity,and the expression levels of HPAProtein and integrinα5β1Protein in tissue in malignant hydatidiform mole group were higher than those in benign hydatidiform mole group(P<0.05).There werePositive correlations between the expressions of HPAProtein and integrinα5β1Protein in both benign and malignant hydatidiform mole tissues(r=0.506,P<0.05;r=0.555,P<0.05).Conclusion:The combined detection of the expressions of HPAProtein and integrinα5β1Protein may become aPredictive indicator for the differential diagnosis of benign hydatidiform mole and malignant hydatidiform mole.

关 键 词：葡萄胎 免疫组织化学 Western blotting法 乙酰肝素酶 整合素Α5Β1 

分 类 号：R714.56[医药卫生—妇产科学]