动态反应釜制备超顺磁性Fe3O4粒子及其DNA提取  

Fabrication of superparamagnetic Fe3O4 particles in a dynamic reaction kettle and their application in DNA extraction

作　　者：盛浩原 苑金磊 刘长霞[1] SHENG HaoYuan;YUAN JinLei;LIU ChangXia(College of Life Science and Technology,Beijing University of Chemical Technology,Beijing 100029,China)

机构地区：[1]北京化工大学生命科学与技术学院

出　　处：《北京化工大学学报（自然科学版）》2019年第6期45-50,共6页Journal of Beijing University of Chemical Technology(Natural Science Edition)

基　　金：厦门市产学研协同创新及科技合作项目(3502Z20172020)

摘　　要：使用动态反应釜制备得到磁性粒子,与静态反应釜相比单次制备量提高20倍;通过扫描电子显微镜(SEM)、傅立叶红外光谱(FT-IR)、X射线衍射(XRD)、振动样品磁强计(VSM)等手段对产物进行表征,证明获得了粒径200 nm左右的单分散Fe3O4粒子,并具有超顺磁性;对其表面进行SiO2包覆,获得具有良好分散性的Fe3O4@SiO2粒子。研究发现Fe3O4@SiO2对DNA提取具有可重复利用性,并且质粒DNA吸附到Fe3O4@SiO2上后可直接加入聚合酶链式反应(PCR)体系作为扩增模板。Monodispersed 200 nm superparamagnetic Fe3O4 particles have been prepared in a dynamic reaction kettle under optimized conditions,giving a product yield 20 times that of a static reaction.The product was characterized by SEM,FT-IR,XRD and VSM.The surfaces of Fe3O4 particles were coated with SiO2 to obtain Fe3O4@SiO2 particles,with high dispersibility.Fe3O4@SiO2 particles could be used repeatedly for plasmid DNA extraction leading to reduced costs.Plasmid DNA on Fe3O4@SiO2 could be used as DNA templates for PCR.

关 键 词：动态反应釜 超顺磁性Fe3O4粒子 Fe3O4@SiO2 DNA提取 重复利用 

分 类 号：TQ138.1[化学工程—无机化工]