透射电镜和光镜联合应用于细胞观察  

The Application of Correlative Electron Microscopy and Light Microscopy on the Cells Research

作　　者：李红梅[1] 张勤奋[1] 李茵茵[1] 张楚英[1] 张子健[2] 张以顺[1] LI Hongmei;ZHANG Qinfen;LI Yinyin;ZHANG Chuying;ZHANG Zijian;ZHANG Yishun(School of Life Sciences,Cancer Center,Sun Yat-sen University,Guangzhou 510275,China;State Key Laboratory of Oncology in South China,Cancer Center,Sun Yat-sen University,Guangzhou 510275,China)

机构地区：[1]中山大学生命科学学院,广州510275 [2]中山大学华南肿瘤学国家重点实验室,肿瘤防治中心,广州510275

出　　处：《实验室研究与探索》2019年第11期18-20,65,共4页Research and Exploration In Laboratory

基　　金：国家自然科学基金资助项目(31570736)

摘　　要：在光学显微镜下获得细胞的全视野荧光图像及位置信息,并在培养皿进行原位细胞透射电镜样品制备、超薄切片及染色,最后在透射电镜下获得目标细胞的超微结构信息。采用该方法成功获得处于细胞分裂M期细胞的显微结构,既有完整细胞的荧光图像,又有局部的超微结构的高分辨图像。该方法能够用在较高分辨率下分析亚细胞结构分布以及特定结构的动态变化。Bridging these two modalities,correlative light and electron microscopy(CLEM),can complement each other very well.The cells were cultured in a grid-labeled dish.The structure of molecules within living cells and localization of interested cells were obtained using optical microscopy.Then in situ cells were prepared for electron microscopy(EM).Finally,nanometer resolution images of cellular structures in fixed cells was obtained by EM after ultrathin section and stained.Using this method,the structures of a metaphase cells were successfully obtained,which contained both fluorescent images of complete information and high-resolution images of cellular structures.This method can be used for analysis the location of interested substructures in cells and dynamic events in the context of specific cellular structures at high resolution.

关 键 词：透射电镜 光学显微镜 光电联用 

分 类 号：Q271[生物学—细胞生物学] Q6-4