基于微流控芯片的免疫荧光判读仪的研制  

Development of Immunofluorescence Reader Based on Microfluidic Chip

作　　者：王雷 刘江 段志超 付双岁 WANG Lei;LIU Jiang;DUAN Zhichao;FU Shuangsui(Tianjin Paipudaye Instrument Technology Co.,Ltd.,Tianjin,300400)

机构地区：[1]天津派普大业仪器科技有限公司

出　　处：《中国医疗器械杂志》2019年第6期422-424,428,共4页Chinese Journal of Medical Instrumentation

摘　　要：该文简介了微流控免疫荧光判读仪的技术原理,然后从系统、光学、硬件和软件算法等方面介绍了判读仪的研制方法。首先设计判读仪的光电传感器,然后在对传感器扫描的数据进行滤波、寻找曲线峰等算法进行了研究,最后获得样品液的定量浓度结果。该文对临床中的总前列腺特异性抗原(PSA)标准样品进行定量准确性和重复性实验测试,结果表明判读仪与韩国NanoEnTek公司的判读仪相关系数大于99%,变异系数(CV)小于10%。This paper introduces the technical principle of microfluidic immunofluorescence interpreters,and then introduces the development method of reader from the aspects of the system,optics,hardware and software algorithms.Firstly,the photoelectric sensor of the reader is designed,and then the software algorithms such as filtering the data scanned by the sensor and finding the curve peak are researched.Finally,the quantitative concentration result of the sample liquid is obtained.In this paper,a quantitative experimental test on clinical PSA standard samples was performed,and the results indicate the correlation coefficient between the reader and the FREND reader of NanoEnTek was more than 99%,and the CV is less than 10%.

关 键 词：微流控 免疫荧光 定量测试 

分 类 号：R446.5[医药卫生—诊断学]